Chromium has been proposed to be an essential trace element useful in the treatment of diabetes, and several chromium(III) compounds have been used as nutritional supplements. However, the fate of the chromium from nutritional supplements has been investigated solely by techniques, such as radio-labeling, which allow only the chromium atom to be traced, not its chemical form. This investigation...

Our study aimed to investigate the co-localization and protein-protein interactions between ezrin and p65 in human breast cancer cells. Liquid chromatography-mass spectrometry (LCMS) was used to uncover novel protein interactions with ezrin in MDA-MB-231 cells. Endogenous co-immunoprecipitation was used to validate protein-protein interactions between ezrin and p65 in MDA-MB-231. Exogenous inte...

Terminalia catappa and subspathulata are two species of the Combretaceae family medium to large forest trees. The fruits T. known for edible nuts commonly as tropical almonds due their similarity in taste with commerce. Therefore, chemical profiles were examined ascertain potential value food or health uses. Gas chromatography-mass spectrometry (GCMS) ultrahigh-pressure liquid chromatography-el...

In this study, it was investigated whether different bee breeds prefer plant sources to collect propolis. For purpose four honey race (Apis mellifera caucasica, A. m. carnica, syriaca and anatoliaca) naturally have been in Turkey were placed the same isolated apiary; Propolis harvested from these races. chemical contents of alcoholic extractions propolis analyzed by liquid chromatography-tandem...

PURPOSE To investigate the potential use of accelerator mass spectrometry (AMS) in the study of the clinical pharmacology of imatinib. EXPERIMENTAL DESIGN Six patients who were receiving imatinib (400 mg/d) as part of their ongoing treatment for chronic myeloid leukemia (CML) received a dose containing a trace quantity (13.6 kBq) of (14)C-imatinib. Blood samples were collected from patients b...

The conversion of deuterium-labeled ceramide to cerebroside has been studied. A mixture of N-(2’+hydroxy [4’,4’,5’,5’-2H4]hexadecanoyl) D-eryfhYO-1,3-dihydroxy-2amino-4-frans-[4,5-*H2]octadecene and N-(2’-D-hydroxy hexadecanoyl) D-eryfhro-l,3-dihydroxy-2-amino-4-frans-[33Hl]octadecene was incubated with mouse brain microsomes and a UDP-galactose regenerating system. Both galactosyl ceramide and...

Biomedical Research Models, Inc. In vivo receptor occupancy (RO) is a valuable tool used in preclinical drug development to confirm target engagement and faci litate selection of compounds for further development. We have been using radioligand-based RO as means of measuring target engagement in the rat brain. In this approach, compounds of interest are peripherally injected prior to intravenou...

This paper reports on the vinca alkaloid produced by a novel Nigrospora sphaerica isolated from Catharanthus roseus. Through liquid chromatography-mass spectrometry (LCMS), only the crude mycelia extract of this fungus was positive for determination of vinblastine. This vinca alkaloid was then purified by using high-performance liquid chromatography (HPLC) and tested for cytotoxicity activity u...