RNase and DNase activities were studied in seven fungi of the subdivisions Ascomycotina, Zygomycotina and Basidiomycotina during their autolysis, and extracellular and intracellular RNase and DNase were found. RNase specific activity reached higher levels than DNase specific activity in the culture liquid and mycelial extract, except in Aspergillus nidulans. Generally maximal RNase specific act...

OBJECTIVES To evaluate BD MGITTM TBc® technique for identifying the Mycobacterium tuberculosis complex using isolates obtained in liquid and solid media. METHODS A descriptive study was conducted in which 117 isolates were analyzed by the immune-chromatography technique obtained from solid and liquid cultures to identify the Mycobacterium tuberculosis complex. The kappa coefficient was calcul...

BACKGROUND The isolation of non-tuberculous mycobacteria (NTM) has been increasing in South Korea. To date, however, the cause of this increase has not been determined, and it remains unclear whether the use of liquid media has contributed to this increase. The aim of this study was to evaluate the factors associated with NTM isolation and the impact of liquid media on NTM culture. METHODS My...

The effect of different concentrations of the anionic surfactant sodium dodecyl sulfate (SDS) on biodegradation of diesel oil was assessed during 32 days at 10 degrees C, under simulated environmental conditions, in liquid culture and in an alpine soil. Low SDS concentrations (50-100 mg l-1) significantly enhanced oil biodegradation by a psychrotrophic inoculum in liquid culture, whereas higher...

A standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an Aminex HPX-87 column. The acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 N NaOH. The presence of fumaric acid in culture extracts of Peptostreptococ...

this study was conducted with the main prupose of investigation the effects of culture medium and the concentration of growth regulators on proliferation rate, rooting and the acclimatization of gisela 6 rootstock at khorasan razavi agriculture and natural resource research center during 2011-2012. in this experiment, the proliferation and rooting were performed in six kinds of culture medium (...

Fischer, 0.: Enhancement of Propagation of Mycoplasma hyopneumoniae by Culture in a Biphasic Medium. Acta vet. Bmo, 1995,64:243-247. Mycoplasma hyopneumoniae (strain J) was propagated in a liquid and a biphasic media. The solid phase of the biphasic medium was obtained by the addition of 1.9% or 0.19% of agar to the liquid medium. Compared with the liquid medium, a more rapid propagation of myc...