In the present study, we show that recombinant human interleukin-1 beta (rhIL-1 beta), which has no effect on the proliferation of human progenitor cells, has synergistic effects on the expansion of human progenitor cells induced by rhIL-3 in liquid cultures. The synergistic effects of rhIL-1 beta with rhIL-3 were observed in liquid cultures using not only fresh bone marrow mononuclear cells, b...

Linum spp. (Linaceae) has been shown to accumulate cytotoxic lignans such as podophyllotoxin and 6-methoxypodopyllotoxin. Therefore, plant cell and tissue cultures of Linum spp. may be possible alternative sources for the lignan production. In this study, we focused on the production of lignans by root cultures of Linum spp. Hairy root cultures of L. nodiflorum, L.album and L. austriacum and ro...

Ceratocystis coerulescens RWD 451 (Ascomycetes) was cultivated on defined synthetic culture medium containing glucose (3.0% ), asparagine (0.1% ), mineral salts and thiamine. 6 weeks oid cultures produced methyl heptenyl compounds, citronellol. citronellyl acetate, dihydro-farnesol and a hitherto unknown monoterpene alcohol. By GC/MS, 'H and l3C NMR spectroscopy, this alcohol could be identifie...

background diazinon is one of the most widely used organophosphrous pesticides in the world may affect animals and human. objectives this study was done to assess whether pseudomonas plecoglossicida can grow on diazinon as the sole carbon metabolic source in laboratory and agricultural soil. materials and methods serial dilutions of pure and commercial diazinon in minimal salt medium were made ...

A radioimmunoassay that measures a specific secretory tuberculoprotein was used to detect Mycobacterium tuberculosis in 9 of 30 liquid cultures of sputum. The accumulation of immunoreactive material in liquid cultures containing isoniazid was shown to reflect in vitro susceptibility of mycobacteria to the antibiotic effects of the drug.

Organotypic cultures of primary human airway epithelial cells have been used to investigate the morphology, ion and fluid transport, innate immunity, transcytosis, infection, inflammation, signaling, cilia, and repair functions of this complex tissue. However, we do not know how closely these cultures resemble the airway surface epithelium in vivo. In this study, we examined the genome-wide exp...

In synchronized continuous cultures of Saccharomyces cerevisiae CBS 8066, the production of the extracellular invertase (EC 3.2.1.26) showed a cyclic behavior that coincided with the budding cycle. The invertase activity increased during bud development and ceased at bud maturation and cell scission. The cyclic changes in invertase production resulted in cyclic changes in amounts of invertase l...

zinc and iron nano-oxides (100 ppb) were promoted the hypericin and hyperforin production in hypericum perforatum cell suspension culture. high performance liquid chromatography method was used for detectection and identification of hypericin and hyperforin in h. perfuratum cell suspension cultures elicited with different concentrations of zinc and iron nano-oxide (0, 50, 100 and 150 ppb) after...