We studied whether nucleoid exclusion contributes to the segregation and retention of Tsr chemoreceptor clusters at the cell poles. Using live time-lapse, single-cell microscopy measurements, we show that the single-cell spatial distributions of Tsr clusters have heterogeneities and asymmetries that are consistent with nucleoid exclusion and cannot be explained by the diffusion-and-capture mech...

Live working on power distribution lines is an effective way to improve the reliability of supply. The aerial lift device with insulated boom often used as a platform carry out live working, which difficult in mountainous areas, paddy fields, and other complex environments. At same time, requires highly skilled operators, lot personal security measures, not conducive application some developing...

This paper presents analysis and comparative study of a novel high-torque three-phase switched reluctance motor (SRM) with magnetically isolated stator segments. In the proposed SRM, each segment has a concentric winding located on the center body of it and two diametrically opposite windings which form the motor phase. There are four salient poles in the stator segment. Two of them share their...

Activity of the sliding motor Eg5 and coordinated microtubule dynamics are both essential for mitotic spindle pole separation. It is still a matter of controversy if changes in microtubule dynamics can compensate inhibition of Eg5 activity and re-enable bipolarization. Using a consistent live cell-imaging approach, we show that perturbation of microtubule dynamics can compensate inhibition of E...

Even single-celled organisms, such as Escherichia coli, possess a far from random internal organization, as the cytoplasm is a crowded, heterogeneous environment. Some proteins preferentially locate at the cell poles (e.g. those involved in chemo-taxis), while others, e.g. involved in gene expression, locate at mid-cell, within a structure known as nucleoid. Recent live cell microscopy measurem...

During mitosis, the Drosophila protein Asp binds and focuses microtubule minus ends at the spindle poles, and maintains their attachment to the centrosomes. Two groups of researchers now provide insights into how the protein achieves these functions (1, 2). asp was one of the fi rst genes to be iden-tifi ed as being required for correct assembly of the Drosophila mitotic spindle (3), and mutati...

Tipulid spermatocytes form normally functioning bipolar spindles after one of the centrosomes is experimentally dislocated from the nucleus in late diakinesis (Dietz, R., 1959, Z. Naturforsch., 14b:749-752; Dietz, R., 1963, Zool. Anz. Suppl., 23:131-138; Dietz, R., 1966, Heredity, 19:161-166). The possibility that dissociated pericentriolar material (PCM) is nevertheless responsible for the for...

We review results obtained after fluorescent actin and myosin II probes were microinjected into interphase and prophase PtK2 and LLC-PK tissue culture cells to follow the changing distribution of these cytoskeletal proteins in the live cells during division. The fluorescent probes first begin to assemble into the future furrow region during mid-anaphase before any sign of initial contractions. ...

New information has been obtained recently regarding microtubule organization in Xenopus extract spindles. These spindles assemble in vitro by chromatin-mediated microtubule nucleation and consist of randomly interspersed long and short microtubules with minus ends distributed throughout the spindle. Fluorescence speckle microscopy has led to the proposal that the Xenopus steady-state spindles ...