Microsomes are derived mostly from endoplasmic reticulum and are an ideal target to investigate compound metabolism, membrane-bound enzyme functions, lipid-protein interactions, and drug-drug interactions. To better understand the molecular mechanisms of the liver and its diseases, mouse liver microsomes were isolated and enriched with differential centrifugation and sucrose gradient centrifuga...

Autoimmune hepatitis (AIH), a liver disorder affecting both children and adults, is characterized by inflammatory liver histology, elevated transaminase levels, circulating nonorganspecific autoantibodies, and increased levels of immunoglobulin G, in the absence of a known etiology. Two types of AIH are recognized according to seropositivity: smooth muscle antibody and/or antinuclear antibody d...

conclusions the lee is a common finding among kidney transplant recipients. serial monitoring of aminotransferases, particularly alt, should be performed in all patients after kidney transplantation. patients and methods liver enzymes and other biochemical parameters were measured in all recipients. patients were divided into three groups, according to laboratory test time since transplantation...

1. The activities of microsome fractions from the liver of adult and 5-day-old rats for the incorporation of [(14)C]phenylalanine into protein were similar in the presence and absence of polyuridylic acid. 2. The activity of a light-microsome fraction from adult liver was greater than that of a heavy-microsome fraction, and the light-microsome fraction was also more markedly stimulated by the p...

Although the synthesis of specific soluble proteins has rarely, if ever, been unequivocally demonstrated to occur in cell-free preparations of mammalian cells, the capacity for the uptake of amino acids into peptide linkage in 'non-specific' protein is less readily destroyed by damage to the cell. Thus the incorporation of labelled amino acids into the acid-precipitable protein of tissue homoge...

BACKGROUND AND OBJECTIVES The frequency of Type 1 diabetes (T1D)-related autoantibodies was determined in children with autoimmune hepatitis. However, the incidence of autoimmune hepatitis related autoantibodies in children with T1D has been poorly investigated. The aim of the present cross sectional prospective study was to determine the occurrence of autoimmune hepatitis-related autoantibodie...

Palmitoyl-coenzyme A (palmitoyl-CoA) hydrolase was obtained from rat kidney in an electrophoretically homogeneous form. The enzyme associated with carboxylesterase activity was purified by acetone precipitation of microsomes, followed by successive chromatographies on DEAE-cellulose, phenyl-Sepharose, and Sephadex G-100 gel. The two activities in rat kidney were associated as judged by their co...