Representative amino acid sequences of the RNA-dependent RNA polymerases of all groups of positive-strand RNA viruses were aligned hierarchiacally, starting with the most closely related ones. This resulted in delineation of three large supergroups. Within each of the supergroups, the sequences of segments of approximately 300 amino acid residues originating from the central and/or C-terminal p...

Purified BLRV RNA was converted to cDNA and cloned into pT7T3 19U phagemid. The nucleotide sequence of a putative bean leafroll luteovirus coat protein gene was determined from the clones pBL9, pBL29 and pBL32 by the chain termination method (1). Sequence analysis reveals a 588 bp open reading frame and an internal 426 bp ORF (Fig. 1). The large ORF is equivalent to a protein of Mr 21.98 kDa, t...