Distribution and modulation of rabbit lymphocyte phytohaemagglutinin acceptors and concanavalin A acceptors during activation of rabbit lymphocytes have been examined by electron microscopy. Two types of cell surface acceptors have been tentatively identified, lectin binding acceptors that do not modulate, and receptors that are endocytosed when blast transformation is stimulated. All of the ce...

Lymphocyte function in rheumatoid arthritis has been assessed by the mixed lymphocyte culture assay. In contrast to previous studies, no difference was found between lymphocytes from healthy volunteers and patients with rheumatoid arthritis. An occasional patient's lymphocytes failed to respond on a given day, but were normal on restudy. All 44 arthritic patient's lymphocytes underwent blast tr...

Lymphocytes respond to mitogens that stimulate proliferation by increasing theirs metabolic activity. In this study we investigate L-Glutamine and L-Leucine uptake as markers of cell response to Concavalina A (ConcaA) stimulation, using a high-resolution flow technique. We found that lymphocytes induced to blast transformation enhanced rate and efficiency of amino acid uptake during cell prolif...

O. P. SHARMA The cardinal immunological defect in sarcoidosis is depression of delayed-type hypersensitivity which is readily demonstrated by skin tests using tuberculin, Candida albicans, or the chemical dinitrochlorobenzene (DNCB). It is also known that in vitro culture of lymphocytes from sarcoidosis patients fail to undergo blastic transformation in response to phytohaemagglutinin (PHA). We...

Blast cells which were derived from rat lymphocytes by stimulation with phytohemagglutinin (PHA), concanavalin A (Con A), or pokeweed mitogen (PWM) transformed within 2-3 days into a new type of lymphocytes when plated without mitogen on embryo fibroblast monolayers. These lymphocytes were termed secondary lyrophocytes. Upon addition of PWM to PWM-secondary lymphocytes a marked adherence to fib...

1. Phytohaemagglutinin stimulates the transformation into blast cells of human lymphocytes incubated in vitro. This transformation is accompanied by an increase in the incorporation of [(14)C]leucine into protein and [(3)H]uridine into RNA. 2. The incorporation of [(14)C]leucine by cultures grown in the presence or absence of phytohaemagglutinin is inhibited to the same extent by cycloheximide,...

Quinine- or quinidine-induced thrombocytopenic purpura is caused by synthesis of an immunoglobulin (Ig)G antibody, which caused platelet damage in the presence of the offending drug. The nature of the antigenic stimulus has been examined by measuring incorporation of [3H]thymidine into DNA during lymphocyte transformation to blast cells in the presence of the drug. Although patients' lymphocyte...

UNLABELLED The purpose of research is to study the influence of plant mitogens and antigens of the water-salt extract from the bodies of Hirudo verbana, H. medicinalis, H. orientalis on the reaction of lymphocyte blast-transformation and the synthesis of pro-inflammatory cytokines (IL-1β, IL-8, TNF-α) in patients' cell culture supernatants before and after hirudotherapy. RESEARCH METHODS the ...