introduction: prostate specific antigen (psa) is one of the most sensitive markers for diagnosis of prostate cancer. immunoradiometric kit (irma) is a common and sensitive method for determination of psa in clinical laboratories. this kit has four major components: solid phase coated with monocolonal antibody, pair antibody labelled with i-125, series of standards in different ranges of concent...

A micro-radioimmunoassay of 125I-labeled digoxin is described in which 10-mul rather than 50-mul aliquots of sera are required. The method is a modification of a simplified, rapid, and accurate procedure, which is commercially available as a kit. There is excellent correlation between results by these two methods, in which lyophilized digoxin standards and insoluble antibody polymer are used. T...

  Introduction: Prostate specific antigen (PSA) is one of the most sensitive markers for diagnosis of prostate cancer. Immunoradiometric kit (IRMA) is a common and sensitive method for determination of PSA in clinical laboratories. This kit has four major components: solid phase coated with monocolonal antibody, pair antibody labelled with I-125, series of standards i...

An RT-PCR based microchip test system for the detection of SARS-CoV-2 offers pre-loaded and lyophilized reagents in microchip. However, 30- 48-microwell formats being miniaturized performing 1.2 μl reaction, seek visual attention during sample addition. Therefore, adding colorants as color indicator matrix microchips or to master mix can impart not only user-friendliness task liquid handling bu...

Radiolabeled Microspheres of human serum albumin (MSA) is of great importance in the analysis of regional lung perfusion. Preparation of MSA kit has been installed in our Division recently is carried out in two stages: 1. Production of albumin sphere particles with suitable sizes by using the spinning disk technique. 2. Formulation of MSA as a radiopharmacutical kit. So it can be labelled...

Lung scanning has been recognized as a clinically useful technique for portraying macroscopic pulmonary perfusion defects since it was first reported by Wagner in 1964 (1 ). A number of radiopharmaceuticals have been proposed to assess pulmonary perfusion. Although 1 3 1 I imposes many limitations as a radiotagging agent, the use of iodinated macroaggregated albumin (I-MAA) has received wide ac...

The aim of this work was to prepare a kit of Tc-ceftizoxime (Tc-CFT), with stability and biological activity preserved, able to identify a septic focus (E. coli) in the experimental infection model in rats. The preparation of the CFT kit involved the use of lyophilized solutions containing the antibiotic ceftizoxime and the sodium dithionite reducing agent (6.0 mg/mL). After lyophilization, the...

The aim of the work was to implement risk management strategies in manufacturing and use medical products for vitro diagnostics by example experimental series reagent kit “Lyophilized erythrocyte antigenic tularemia diagnosticum”. Materials methods. We tested panel To carry out identification, assessment analysis risks regarding considered product, failure mode effects (FMEA) proposed adapted u...

Rapid developments in the field of medical imaging have opened new avenues for the use of positron emitting labeled microparticles. The radioisotope used in our research was (68)Ga, which is easy to obtain from a generator and has good nuclear properties for PET imaging. Methods. Commercially available macroaggregated albumin (MAA) microparticles were suspended in sterile saline, centrifuged to...

Three laboratories compared their routine steroid binding assays with the Abbott estrogen receptor-enzyme immunoassay (ER-EIA) to determine ER in breast cancer cytosols. Each laboratory was first trained to use the ER-EIA kit and then performed routine proficiency panels to determine assay reproducibility. The frozen panels prepared from MCF-7 cytosol produced good intraassay results but the be...