Purified potato tuber (Solanum tuberosum L. cv Bintie) mitochondria contain soluble, highly latent NAD(+)- and NADP(+)-isocitrate dehydrogenases, NAD(+)- and NADP(+)-malate dehydrogenases, as well as an NADPH-specific glutathione reductase (160, 25, 7200, 160, and 16 nanomoles NAD(P)H per minute and milligram protein, respectively). The two isocitrate dehydrogenase activities, but not the two m...

Malate dehydrogenases are widely distributed and alignment of the amino acid sequences show that the enzyme has diverged into 2 main phylogenetic groups. Multiple amino acid sequence alignments of malate dehydrogenases also show that there is a low degree of primary structural similarity, apart from in several positions crucial for nucleotide binding, catalysis, and the subunit interface. The 3...

Monospecific antibodies to glyoxysomal, mitochondrial, and cytosolic I malate dehydrogenase were used for the fluorescence immunohistochemical localization of these isoenzymes in dark-grown watermelon (Citrullus vulgaris Schrad.) cotyledons. It was demonstrated that, with cell organelles isolated by sucrose density gradient centrifugation, antibodies to glyoxysomal malate dehydrogenase were spe...

1. NADP-malate dehydrogenase and ;malic' enzyme in maize leaf extracts were separated from NAD-malate dehydrogenase and their properties were examined. 2. The NADP-malate dehydrogenase was nicotinamide nucleotide-specific but otherwise catalysed a reaction comparable with that with the NAD-specific enzyme. By contrast with the latter enzyme, a thiol was absolutely essential for maintaining the ...

The activity of cytoplasmic malate dehydrogenase (EC 1.1.1.27) of livers of young (9-10 weeks) and old (60-70 weeks) rats decreases after adrenalectomy and increases after administration of cortisone to adrenalectomized rats. These changes are significantly lower in old rats. The induction by cortisone is inhibited by actinomycin D. Adrenalectomy decreases and cortisone increases the activity o...

Malate oxidation supported C(2)H(2) reduction by bacteroids isolated from Sesbania rostrata stem nodules. Optimal activity reached 7.5 nanomoles per minute per milligram of dry weight and was in the same order of magnitude as that observed with succinate but always required a lower O(2) tension. Malate dehydrogenase (EC 1.1.1.37), purified 66-fold from bacteroids, actively oxidized malate (K(m)...

In an attempt to elucidate the relationships between some pyridine nucleotide-dependent dehydrogenases in Bacillus subtilis, such as alanine dehydrogenase, malate dehydrogenase, lactate dehydrogenase, and glutamate dehydrogenase, these enzymes are being purified and characterized with respect to their chemical, serological, and enzymic properties. In two previous papers (1, 2), the purification...

In an attempt to elucidate the relationships between some pyridine nucleotide-dependent dehydrogenases in Bacillus subtilis, such as alanine dehydrogenase, malate dehydrogenase, lactate dehydrogenase, and glutamate dehydrogenase, these enzymes are being purified and characterized with respect to their chemical, serological, and enzymic properties. In two previous papers (1, 2), the purification...

The mitochondrial tricarboxylic acid cycle enzyme malate dehydrogenase was purified from Saccharomyces cerevisiae, and an antibody to the purified enzyme was obtained in rabbits. Immunoscreening of a yeast genomic DNA library cloned into a lambda gt11 expression vector with anti-malate dehydrogenase immunoglobulin G resulted in identification of a lambda recombinant encoding an immunoreactive b...

NADH:ubiquinone reductase (complex I) of the mitochondrial inner membrane respiratory chain binds a number of mitochondrial matrix NAD-linked dehydrogenases. These include pyruvate dehydrogenase complex, a-ketoglutarate dehydrogenase complex, mitochondrial malate dehydrogenase, and &hydroxyacylCoA dehydrogenase. No binding was detected between complex I and cytosolic malate dehydrogenase, gluta...