Matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) is a common molecular modality used to characterise the abundance and spatial distribution of lipids in situ. There are several technical challenges predominantly involving sample pre-treatment preparation which have complicated analysis clinical tissues by MALDI-MSI. Firstly, embedding samples optimal cutting tem...

An atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) source coupled to Fourier transform ion cyclotron resonance mass spectrometry (FT ICR MS) under UV laser and solid matrix conditions has been demonstrated to analyze a variety of labile oligosaccharides including O-linked and N-linked complex glycans released from glycoproteins. Spectra were acquired by both AP MALDI...

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) -based identification of bacteria and fungi significantly changed the diagnostic process in clinical microbiology. We describe here a novel technique for bacterial and yeast deposition on MALDI target using an automated workflow resulting in an increase of the microbes' score of MALDI identification. We ...

e17544 Background: High-grade serous ovarian cancer (HGSOC) can be separated by gene expression profiling into four molecular subtypes with clear correlation of the clinical outcome. However, these signatures have not been implemented in practice to stratify patients for targeted therapy. This is mainly due a lack easy, cost-effective and reproducible methods, as well high heterogeneity HGSOC. ...

This article presents the utility of mass spectrometry with a MALDI ionization source in natural products analysis. The advantages and drawbacks of this technique for natural products analyses will be presented and discussed. In addition, the structural determination of secondary metabolites using MALDI-MS/MS will be explored, which can guide MALDI experimental methods and stimulate new researc...

Matrix Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry (MS) with a computer-controlled xy-stage in the ionization source can be used to detect the presence, approximate amount and location of small molecules, lipids, peptides and proteins in tissue samples through in vitro molecular imaging, often referred to as MALDI imaging. Both MALDI Time-of-flight/Time-of-flight (MALDI-TOF/T...