Rapidly progressing proteomics techniques have been widely adopted in most areas of biology and medicine. In neurology and neuroscience, many applications of proteomics have involved neurotoxicology and neurometabolism, as well as in the determination of specific proteomic aspects of individual brain areas and body fluids in neurodegeneration. Investigation of brain protein groups in neurodegen...

Endothelins (ETs) are a family of potent vasoactive peptides found in at least three distinct isoforms: ET-1, ET-2, and ET-31}. Two major subtypes of endothelin receptors, termed ETAand ETB, have been identified in various target cells2). It is well known that ETs act on these G protein-coupled receptors and exert multiple pharmacological effects3). As the development of potent and selective en...

Pancreatic-ductal-adenocarcinoma (PDAC) is amongst the most lethal malignancies, mainly because of its metastatic spread and multifactorial chemoresistance. Since c-Met is a marker of pancreatic-cancer-stem-cells (CSC), playing a key role in metastasis and chemoresistance, this study evaluated the therapeutic potential of the novel c-Met/ALK inhibitor crizotinib against PDAC cells, including th...

the proline-rich β-casein was digested in vitro with trypsin, and the oligopeptides producedwere then isolated by rp-hplc and subsequently identified by amino acid analysis and ion massspectrometry. the peptide fractions from the complete digestion were then treated with purified x-prolyldipeptidyl peptidase (x-pdp) extracted from lactobacillus casei ssp. casei llg. two bitter peptides (f53-97a...

Since prolonged severe hypoglycemia could lead to permanent neurological sequelae, it must be treated immediately. But at the same time, the cause of hypoglycemia should be determined to prevent future recurrence of hypoglycemia preferably at bedside while the patient is still in an emergency room. Most of the cases, this could be done by asking proper questions and taking physical findings in ...

In a series of papers under the common title: “Analytical Methods in Quality Control of Scientific Publications,” cases of undisputable breaches of publication ethics and breaches of acceptable rules in the publication of scientific information have been presented. Clear cases of fraud, falsification by some authors, and unqualified review of papers by reviewers and editors were presented in: A...

We analysed solid dose formulations retrieved from an amnesty bin at a London dance venue, into which visitors were required to discard illicit drugs and into which security staff place substances found during searches. We obtained a Home Office licence to analyse the contents of the bin, which were removed in the presence of a police officer and sealed in evidence bags. The data reported are f...

The diverse cellular mechanisms that sustain the life of living organisms are carried out by numerous biomolecules, such as DNAs, RNAs, and proteins. During the past decades, significant research efforts have been made to sequence the genomes of various species and to search these genomes to track down genes that give rise to proteins and noncoding RNAs (ncRNAs) [1], [2]. As a result, the catal...

Peter Derrick’s flattering invitation to comment on this mass spectrometry landmark was followed immediately by a requirement for brevity, obviously impossible in discussing the impact of EJMS. But seeing the comprehensive draft of my good friend Hans-Friedrich Grützmacher, I decided to concentrate instead on the importance of European leadership in mass spectrometry to the success of the whole...

The Anti-Brownian ELectrophoretic trap (ABEL trap) is a new device that allows a user to trap and manipulate fluorescent objects as small as 20 nm freely diffusing in solution. We describe in detail how to build an ABEL trap. CHALLENGES IN NANOMANIPULATION One of the outstanding challenges of nanotechnology is to develop a means to trap and manipulate individual molecules in solution. To study ...