Madin-Darby Canine Kidney (MDCK) cells are widely utilized as a substrate for influenza virus isolation and propagation due to the high yields of virus. Here we compared the conventional MDCK cell line, MDCK-SIAT1 and MDCK-London for viral production, cell survival, and suitability in testing antivirals using six influenza strains including two H1N1 (pandemic and epidemic strains), three H3N2 a...

Background and Aims: Influenza A viruses are important pathogens for humans especially in pandemic episodes. Two adamantane derivates, amantadine and rimantadine, are used for prophylaxis and treatment of influenza A virus infections. However, single amino acid substitutions in the M2 transmembrane domain which lead to amantadine resistance of these viruses occur at residues 26, 27, 30, 31 or 3...

background and aims: influenza virus nucleoprotein (np) has the capacity to be used as subunit vaccine, but little is known about the impact of different cultures on its structure. in the present study we aimed to evaluate and compare the isoelectric focusing (ief) property of extracted viral nucleoproteins derived from madin darby canine kidney (mdck) cell line and embryonated chicken eggs (ec...

background and aims: influenza a viruses are important pathogens for humans especially in pandemic episodes. two adamantane derivates, amantadine and rimantadine, are used for prophylaxis and treatment of influenza a virus infections. however, single amino acid substitutions in the m2 transmembrane domain which lead to amantadine resistance of these viruses occur at residues 26, 27, 30, 31 or 3...

Epithelial cells are important in inflammation and immunity. In this study, we examined if Madin-Darby canine kidney (MDCK) epithelial cells express functional P2X7 receptors, which bind the damage-associated molecular pattern extracellular adenosine 5'-triphosphate (ATP). Reverse transcription (RT)-PCR and immunoblotting revealed the expression of P2X7 in MDCK cells. A flow cytometric assay de...

     The influenza viruses are major etiologic agents of human respiratory infections, and inflict a sizable health and economic burden. This study examines the antiinfluenza virus activity of hydroalcoholic extract of olive leaves (OLHE). Olive leaves were collected from gardens around the city of Shiraz, characterized, dried, ground to powder, and its hydroalcoholic extract was prepared. The ...

Tumor necrosis factor receptor-associated 3 (TRAF3), an adaptor protein, has significant and varying effects on immunity depending cell types. The role of TRAF3 in Madin-Darby Canine Kidney Epithelial (MDCK) resistance to influenza A virus (IVA) remains elusive. In the present study, CRISPR-Cas9 gene editing technology was used construct knockout MDCK cells (MDCK-TRAF3−/−). Hemagglutination ass...

AIM The aim of this study was to isolate Canine parvovirus (CPV) from suspected dogs on madin darby canine kidney (MDCK) cell line and its confirmation by polymerase chain reaction (PCR) and nested PCR (NPCR). Further, VP2 gene of the CPV isolates was amplified and sequenced to determine prevailing antigenic type. MATERIALS AND METHODS A total of 60 rectal swabs were collected from dogs showi...

The Madin-Darby canine kidney (MDCK) cell line, derived from distal tubule/collecting duct, expresses differentiated properties of renal tubule epithelium in culture. We studied the expression of adrenergic receptors in MDCK to examine the role of catecholamines in the regulation of renal function. Radioligand-binding studies demonstrated, on the basis of receptor affinities of subtype-selectiv...

Monoclonal antibodies were prepared against the Madin-Darby canine kidney (MDCK) cell line to identify epithelial cell surface macromolecules involved in renal function. Lymphocyte hybrids were generated by fusing P3U-1 myeloma cells with spleen cells from a C3H mouse immunized with MDCK cells. Hybridomas secreting anti-MDCK antibodies were obtained and clonal lines isolated in soft agarose. We...