Most protocols for yeast subcellular fractionation involve the use of mechanical shear forces to lyse the spheroplasts produced by the enzymatic digestion of the Saccharomyces cerevisiae cell wall. These mechanical homogenization procedures often involve the manual use of devices such as the Dounce homogenizer, and so are very operator-dependent and, in consequence, lack reproducibility. Here, ...

homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free gsh) from baker’s yeast. the study was done to determine the effects of specific parameters of the methods and the ph of the medium on the production of the gsh. the cell disruptions were run at a wide range of those parameters at the suitable...

2 From its very genesis APV has maintained an impeccable reputation for excellence worldwide. That standard is perpetuated in the field of homogenization and high pressure pumps. It's a tradition that dates back to our invention of the homogenizer in the early 1900's. That desire to excel in saving our customers is demonstrated today through innovative design, precision, engineering, quality ma...

Cellulose is the most abundant material in earth, it considered a renewable polymer of cell walls wood and plant cells, their interest use different field purpose extracting namely: cellulose native (cotton), transformation into sheets (paper filter paper), cupric cellulose, zinc (industrial applications), functionalization cellulosic materials printed electronics, textile, treatment, medicine,...

In this paper, the efficiency and uniformity improvement of non-image LED illumination system with collimator, microlens array and condenser are proposed for pico-projector. The LED light is collimated to 18 degree with efficiency 99.36% by collimator. For image uniformity and flux efficiency, the doule-side microlens array and two condensers are adopted to form a homogenizer. With this homogen...

The use of cellulose nanofibers (CNF) as an additive in papermaking is an attractive alternative to improve paper’s strength. However, the costs of CNF production need to be competitive compared to other approaches aimed at reducing mechanical beating. Five different types of CNFs were prepared following different pretreatments: TEMPO-mediated oxidation at basic and neutral conditions, soft aci...