Random ampli®ed polymorphic DNAs (RAPDs) were used to discriminate among 25 Feijoa sellowiana cultivars and accessions. Fifty ten-mer oligonucleotide primers were tested on ®ve accessions. Twenty-two primers, showing clear polymorphic patterns, were chosen to amplify the DNAs of all 25 genotypes; each ampli®cation was repeated three times separately. Only 11 primers were ®nally analysed, yieldi...

Random amplified polymorphic DNA (RAPD) analysis was employed to estimate genetic diversity/genetic similarity among nine wheat (Triticum aestivum L.) genotypes. Out of 25 random 10-mer primers surveyed, 18 primers detected polymorphism among all the wheat genotypes, while 7 primers produced monomorphic pattern. A total of 175 bands were amplified, 60.57% of which were polymorphic. Genetic simi...

ascochyta rabiei is one of the devastaining fungi pathogens of chick-pea (cicer arietinum l.) crops. in the present investigation, forty-two iranian isolates of ascochyta rabiei fungi (including highly, weakly and moderate virulent isolates), whose pathogenisities were determined previously, were studied. polygalacturonase is a main factor in the initiation of ascochyta blight disease. therefor...

trans-4-Hydroxynonenal (HNE) is the major peroxidation product of ω-6 polyunsaturated fatty acids in vivo. Michael addition of the N(2)-amino group of dGuo to HNE followed by ring closure of N1 onto the aldehyde results in four diastereomeric 1,N(2)-dGuo (1,N(2)-HNE-dGuo) adducts. The (6S,8R,11S)-HNE-1,N(2)-dGuo adduct was incorporated into the 18-mer templates 5'-d(TCATXGAATCCTTCCCCC)-3' and d...

Barley powdery mildew, caused by the pathogen Blumeria graminis f. sp. hordei is an important disease of barley (Hordeum vulgare L.). The random amplified polymorphic DNA (RAPD) method was used to detect DNA polymorphism among 7 Pallas near-isogenic lines (NILs) carrying Mla3, Mla12, Mlk, Mlp, Mlat, Mlg and MlLa genes for resistance to B. graminis f. sp. hordei. From among 500 random 10-mer pri...

A new method was developed to detect DNA methylation in the Citrus genome using random amplification coupled with restriction enzyme digestion. Genomic DNA from Citrus grandis (L.) Osb., Poncirus trifoliata (L.) Raf., and their F1 hybrid was amplified using 7 individual 10-mer random primers. Prior to amplification the DNA templates were digested with 2 pairs of restriction endonucleases (HpaII...

Individuals from 33 unrelated Australian families with optic atrophy were screened for 10 different single base alterations in mitochondrial DNA (mtDNA) associated with Leber hereditary optic neuropathy (LHON) using direct polymerase chain reaction amplification of blood spots collected on Guthrie cards. This method using blood spots allows easily accessible screening forLHON mtDNA mutations wi...

morphological and rapd markers were used to investigate the genetic diversity among 21 accessions of pomegranate originating from south eastern tunisia. thirteen morphological traits were studied and results showed significant differences for all morphological characters (p< 0.001). clustering based on fruit traits, using ward’s method, divided the accessions into three main groups. in rapd ana...