The homologous ribosomal systems from Escherichia co2i and Uostridium pasteurionum were compared for their ability to translate f2 RNA, formaldehyde-treated f2 RNA, T4 early messenger RNA, E. coli messenger RNA, and C. pasteurianum messenger RNA in protein synthesis assays in vitro. The E. co2i ribosomal system translated all five of these messengers, while the C. pasteurianum ribosomal system ...

Increasing importance of vaccination during coronavirus disease 2019 (COVID-19) pandemic, several vaccines were developed. Messenger RNA (mRNA) including BNT162b2 (Pfizer, New York, NY, USA; BioNTech, Mainz, Germany), mRNA-1273 (Moderna, Cambridge, MA, USA) have been disclosed side effects such as thrombocytopenia, myocarditis and headache in general. In addition, adverse due to autoimmune resp...

In these last years, we are witnessing the emergence of a new class biopharmaceuticals based on messenger RNA (mRNA). One most promising applications mRNA is its use as vaccines. Many reports, including ours, have demonstrated preclinical efficacy vaccines several advantages over traditional or even DNA Unlike attenuated inactivated vaccines, encodes for specific antigen that will be expressed ...

Background: RNA interference (RNAi) is the mechanism of gene silencing-mediated messenger RNA degradation by small interference RNA (siRNA), which becomes a powerful tool for in vivo research, especially in the areas of cancer. In this research, the potential use of an expression vector as a specific siRNA producing tool for silencing of Bcr-abl in K562 cell line has been investigated. Methods:...

Background: Atrial fibrillation (AF) is the most common complication in patients undergoing cardiac surgery. However, pathogenesis of postoperative AF (POAF) elusive, and research related to this topic sparse. Our study aimed identify key gene modules genes conduct a circular RNA (circRNA)-microRNA (miRNA)-messenger (mRNA) regulatory network analysis POAF on basis bioinformatic analysis.

The major mRNA for adenovirus 2 polypeptide pVIII sediments at 18S as assayed by in vitro translation in the messenger-dependent rabbit reticulocyte lysate system. However, a small amount of messenger activity for pVIII sediments at abut 27S, coincident with the mRNA for 100 K. Isolation and fractionation of poly(A) containing RNA following in vitro translation of 27S 100 K mRNA demonstrated th...

Messenger RNA was prepared from developing sea urchin gastrulae by puromycin release from polyribosomes. Approximately 60% of the total mRNA radioactivity of the postnuclear supernatant was recovered and shown to be free of any other labeled RNA species such as ribosomal and nuclear RNA. The mRNA was examined by hybridization to DNA present in great excess. The mRNA hybridizes almost exclusivel...

background: rna interference (rnai) is the mechanism of gene silencing-mediated messenger rna degradation by small interference rna (sirna), which becomes a powerful tool for in vivo research, especially in the areas of cancer. in this research, the potential use of an expression vector as a specific sirna producing tool for silencing of bcr-abl in k562 cell line has been investigated. methods:...

Gene transcripts are under extensive posttranscriptional regulation, including the regulation of their stability. A major route for mRNA degradation produces uncapped mRNAs, which can be generated by decapping enzymes, endonucleases, and small RNAs. Profiling uncapped mRNA molecules is important for the understanding of the transcriptome, whose composition is determined by a balance between mRN...