background one of the major clinical problems regarding pseudomonas aeruginosa is attributed to metallo-beta-lactamases (mbl). this group of enzymes is a subset of beta lactamases which belong to group b of ambler classification and cause hydrolysis of carbapenems. based on epidemiological studies conducted worldwide, it is proved that prevalence of genes coding mbls in p. aeruginosa species ar...

BACKGROUND & OBJECTIVE The production of carbapenemases is an important mechanism responsible for the carbapenem resistance. A simple and inexpensive testing method for screening of carbapenemase producers is essential. A prospective study was undertaken to detect metallo-beta-lactamases (MBLs) and AmpC beta-lactamases in nonfermentative Gram negative bacteria and to evaluate the various method...

Metallo-β-lactamases (MBLs) are a growing threat to the use of almost all clinically used β-lactam antibiotics. The identification of broad-spectrum MBL inhibitors is hampered by the lack of a suitable screening platform, consisting of appropriate substrates and a set of clinically relevant MBLs. We report procedures for the preparation of a set of clinically relevant metallo-β-lactamases (i.e....

A carbapenem-resistant Pseudomonas stutzeri strain isolated from a Dutch patient was analyzed in detail. This isolate produced a metallo-beta-lactamase (MBL) whose gene, with 43.5% GC content, was cloned and expressed in Escherichia coli. beta-Lactamase DIM-1 (for Dutch imipenemase) was weakly related to other Ambler class B beta-lactamases, sharing <52% amino acid identity with the most closel...

background: infectious by pseudomonas aeruginosa has spread worldwide and metallo-beta-lactamases (mbl) are being reported with increasing frequency. the aim of this study was to investigate the antibiotic susceptibility and distribution of blavim and blaimp genes in p. aeruginosa isolates from zanjan province of iran. methods: a total of 70 p. aeruginosa isolates were identified from patients ...

BACKGROUND One of the major clinical problems regarding Pseudomonas aeruginosa is attributed to metallo-beta-lactamases (MBL). This group of enzymes is a subset of beta lactamases which belong to group B of Ambler classification and cause hydrolysis of carbapenems. Based on epidemiological studies conducted worldwide, it is proved that prevalence of genes coding MBLs in P. aeruginosa species ar...

A total of 87 Acinetobacter baumannii nonrepetitive consecutive clinical isolates were tested for the presence of metallo-beta-lactamases (MBLs). Results of phenotypic assays (MBL Etest, imipenem/imipenem-EDTA combined-disk test, and imipenem/EDTA double-disk synergy test) were negative in all cases, but molecular testing revealed the presence of two bla(VIM-1)-carrying isolates. One isolate ha...

conclusions multidrug resistance was significantly associated with mbl production in p. aeruginosa. early detection of mbl-producing p. aeruginosa and hospital antibiotic policy prescription helps proper antimicrobial therapy and avoidance of dissemination of these multidrug resistance isolates. background resistant pseudomonas aeruginosa is a serious concern for antimicrobial therapy, as the c...

Background & Aims: Imipenem is a member of Carbapenem with stability against most -lactamases .It is of particular use in the treatment of infections associated with drug resistant gram negative bacteria harboring ESBL and AmpC genes. The aim of this study was to determine the imipenem resistance in gram negative bacteria causing nosocomial infections and the presence of metallo--lactamases (MB...

Objective. To determine whether the genes whose products are annotated as «MBL-fold metallohydrolase superfamily» related to proteins of metallo-β-lactamase group. Materials and Methods. Sequences 7 were analyzed using following resources: ClustalW, IQ-TREE, iTOL. Selection oligonucleotides for real-time PCR was performed Primer-BLAST resource. The level gene expression assessed PCR. MIC MBC me...