An isolated 5.2-kb fragment of Methylobacterium extorquens AM1 DNA was found to contain a gene cluster involved in methylamine utilization. Analysis of polypeptides synthesized in an Escherichia coli T7 expression system showed that five genes were present. Two of the genes encoded the large and small subunits of methylamine dehydrogenase, and a third encoded amicyanin, the presumed electron ac...

Recent experimental evidence from rainbow trout suggests that gill ammonia transport may be mediated in part via Rhesus (Rh) glycoproteins. In this study we analyzed the transport properties of trout Rh proteins (Rhag, Rhbg1, Rhbg2, Rhcg1, Rhcg2, Rh30-like) expressed in Xenopus oocytes, using the radiolabeled ammonia analogue [(14)C]methylamine, and the scanning ion electrode technique (SIET). ...

Ammonia, methylamine, and pyridine were detected in broth filtrates of a streptomycin-degrading strain of Pseudomonas maltophilia during growth on streptomycin as a sole carbon and nitrogen source. Ammonia and methylamine, quantitatively measured by conversion to chromophores with picryl sulfonic acid, were found to accumulate in broth, whereas pyridine concentration increased in the early stag...

Microorganisms often encode multiple non-orthologous metabolic modules that catalyze the same reaction. However, little experimental evidence actually demonstrates a selective basis for metabolic degeneracy. Many methylotrophs-microorganisms that grow on reduced single-carbon compounds-like Methylobacterium extorquens AM1 encode two routes for methylamine oxidation: the periplasmic methylamine ...

the reaction of ch₃nh₂ with o₂ on the singlet potential energy surfaces (pes) was carried out using the b3lyp, ccsd(t) and g3b3 theoretical approaches along with 6-311++g(3df,3pd) basis set. the suggested mechanism for the title reaction consists of one pre-reactive complex. from the pre-reactive complex, nine types of products, ch2nh+h2o2, ch3nh+ooh, nh2cho+h2o, ch2nho+h2o, nh3+hcooh, ch2nh+h2...

Spectra of clusters formed between lithium atoms and methylamine molecules are reported for the first time. Mass-selective infrared spectra of Li(NH(2)CH(3))(n) have been recorded in both the N-H and C-H stretching fundamental regions. The infrared spectra are broadly in agreement with ab initio predictions, showing redshifted N-H stretching bands relative to free methylamine and a strong enhan...

The excretion of aliphatic amines, methylamine, dimethylamine and trimethylamine in the urine and faeces of rats fed on a control diet and diets supplemented with creatinine, lecithin or choline were measured over a 14 d feeding period. The rats were then killed and concentrations of amines in small and large intestinal contents measured. Adding creatinine to the diet resulted in a significant ...

Methanogenesis has recently been shown to fuel anaerobic oxidation of methane (AOM) within the sulfate-reducing zone marine sediments, coining term “cryptic cycle”. Here we present research on relationship between methanogenesis and AOM in a shallow hypersaline pool (∼130 PSU) southern California coastal wetland. Sediment (top 20 cm) was subjected geochemical analyses, in-vitro slurry experimen...

In the course of feeding experiments in which mono-, di-, and trimethylamines were fed to dogs, it was desirable to determine the urinary excretion of methylamine (monomethylamine). The available methods were found to be cumbersome and not well adapted to the routine analysis of a large number of urines. Weber and Wilson (1) separated ammonia from the amines by the use of yellow mercuric oxide ...