Pseudomonas aeruginosa biofilms are intrinsically resistant to antimicrobial chemotherapies. At present, very little is known about the physiological changes that occur during the transition from the planktonic to biofilm mode of growth. The resistance of P. aeruginosa biofilms to numerous antimicrobial agents that are substrates subject to active efflux from planktonic cells suggests that effl...

BACKGROUND Mex efflux pumps contribute to multidrug resistance in Pseudomonas aeruginosa. Evidencing their expression in clinical isolates would help in rationalizing antibiotic selection. METHODS We have developed a combined phenotypic and genotypic approach for the differential diagnosis of resistance mediated by four major transporters (MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY-OprM). The ...

Of the nine efflux systems of the RND (resistance nodulation cell division) family characterized so far in Pseudomonas aeruginosa, four confer significant resistance to antibiotics when overproduced. These systems, namely, MexAB-OprM (ABM), MexCD-OprJ (CDJ), MexEF-OprN (EFN), and MexXY (XY), are individually able to pump out multiple antipseudomonal compounds, including -lactams (ABM, CDJ, XY),...

Intubated patients frequently become colonized by Pseudomonas aeruginosa, which is subsequently responsible for ventilator-associated pneumonia. This pathogen readily acquires resistance against available antimicrobials. Depending on the resistance mechanism selected for, resistance might either be lost or persist after removal of the selective pressure. We investigated the rapidity of selectio...

BACKGROUND Related Multidrug Resistance (MDR) to efflux pumps is a significant problem in treating infections caused by Pseudomonas aeruginosa (P. aeruginosa). Plant compounds have been identified as Pump Inhibitors (EPIs). In the current study, the potential effect of Berberine and Palmatine as EPIs were investigated on efflux pump inhibition through focusing on different gene patterns in P. a...

The mexCD-oprJ and mexAB-oprM operons encode components of two distinct multidrug efflux pumps in Pseudomonas aeruginosa. To assess the contribution of individual components to antibiotic resistance and substrate specificity, these operons and their component genes were cloned and expressed in Escherichia coli. Western immunoblotting confirmed expression of the P. aeruginosa efflux pump compone...

background: related multidrug resistance (mdr) to efflux pumps is a significant problem in treating infections caused by pseudomonas aeruginosa (p. aeruginosa). plant compounds have been identified as pump inhibitors (epis). in the current study, the potential effect of berberine and palmatine as epis were investigated on efflux pump inhibition through focusing on different gene patterns in p. ae...