Biotin-binding IgG (B-IgG) in human sera was quantified using previously developed F(ab')(2)anti-human IgG-coated multiwell microplates (Muratsugu M. et al., 2003, Biol. Pharm. Bull., 26, 1605--1608). The levels of B-IgG in sera, however, were higher than those we predicted. In this study, we modified the assay using F(ab')2anti-human IgG-coated multiwell microplates and successfully quantified...

This paper describes a method of generating three-dimensional (3D) cell-laden microstructures by applying the principle of origami folding technique and cell traction force (CTF). We harness the CTF as a biological driving force to fold the microstructures. Cells stretch and adhere across multiple microplates. Upon detaching the microplates from a substrate, CTF causes the plates to lift and fo...

In vitro experiments in plastic receptacles are the basis of characterization of new photosensitizers (PSs) for the photodynamic therapy. We recently reported that lipophilic PSs adhere to cell culture microplates in a kinetic-like manner (Engelhardt et al., 2011). In the current study, we examined the interaction and phototoxic effects of the microplate-adhered PS in cancer cells. Therefore, w...

Analysis of static bending, free vibration and buckling behaviours of functionally graded microplates is investigated in this study. The main idea is to use the isogeometric analysis in associated with novel four-variable refined plate theory and quasi-3D theory. More importantly, the modified couple stress theory with only one material length scale parameter is employed to effectively capture ...

In the present study, modelling and vibration analysis of Carbon nanotubes/ fiber/ polymer composite microplates are investigated. The governing equations of the Carbon nanotubes/ fiber/ polymer composite microplates are derived based on first order shear deformation plate theory, rather than other plate theories, due to accuracy and simplicity of polynomial functions. The modified couple stres...

monplace of all disposable labware. Its most common format consists of 96 individual wells arranged in eight rows and 12 columns with dimensions defined by the American National Standards Institute1 through an initiative sponsored by the Society for Biomolecular Sciences in 1996. There are a myriad of microplate types, including different well densities from several wells to several thousand we...