In coffee, bioreactors are the most promising way for scaling-up micropropagation processes, particularly somatic embryogenesis. The availability of an efficient somatic embryogenesis process would allow the rapid mass production of heterozygous materials such as selected Coffea canephora clones and F1 Arabica hybrid varieties. For the last fifteen years, bioreactors (mechanically or pneumatica...

The plant growth regulator diethyl aminoethyl hexanoate (DA-6) has proved highly effective on micropropagation of the medicinal plant purple coneflower (Echinacea purpurea (L.) Moench), however, sharp variation of the effects existed among explants in the same treatment, making the application of DA-6 in micropropagation difficult. In order to clarify factors that influencing the treating resul...

of vegetative propagation methods and insufficient attempts for replenishment of wild stock of different important plant species have contributed to its threatened status. So realizing the threat of extinction and to meet the growing needs, the alternate method for propagation is micropropagation. This technique is used not only for those plants which are difficult to be propagated through conv...

Micropropagation of woody plants and fruit crops constitutes a major success in the commercial application of in vitro cultures. An important aspect to be considered when deriving perennial plants from micropropagation is the maintenance of genetic integrity with regard to the mother plant. In this regard, somaclonal variation has been reported at different levels (morphological, cytological, c...

Agar substrates for in vitro culture are well adapted to plant micropropagation, but not to plant rooting and acclimatization. Conversely, paper-pulp-based substrates appear as potentially well adapted for in vitro culture and functional root production. To reinforce this hypothesis, this study compares in vitro development of nemesia on several substrates. Strong differences between nemesia ro...

The capacity to control developmental and morphogenetic processes in vitro has always been the fascination and primary focus of many ornamental tissue culture studies. Thin cell layers or TCLs were first used to control the development of flowers, roots, shoots and somatic embryos in tobacco pedicels. Since those studies over 30 years ago, TCLs have been successfully used in the micropropagatio...

A protocol for micropropagation of Boswellia ovalifoliolata Bal & Henry (Burseraceae) was developed using cotyledonary nodal explant on Murashige and Skoog modified medium (MS). A comparative study of micropropagation with 6-benzyladenine, kinetin and thidiazuron along with 1-naphthalene acetic acid (0.054 microM) was conducted. The highest shoot multiplication (7.1 +/- 0.2 shoots per node) was...

We investigated the regeneration of two Bulgarian sweet pepper cultivars (‘Hebar’ and ‘Stryama’) on solid and liquid culture medium. Liquid cultures were incubated in temporary immersion RITA ® airlift bioreactors. During a period of 60 days, culture in the liquid medium was associated with significantly increased shoot tip growth, number of lateral shoots, percentage of rooted plants and micro...

Micropropagation is an important technique for rapid and large-scale multiplication of selected plants. The type and concentration of plant growth regulators and physiological status of the explant have profound effect on the efficacy of plant regeneration. However, success of any micropropagation protocol depends upon the percentage survival and successful establishment of plants from in vitro...