RATIONALE Electrospray ionization mass spectrometry (ESI-MS)-based techniques commonly used in oligonucleotide analyses are known to be sensitive to alkali metal adduct formation. Adducts directly impact the sensitivity of MS-based analyses as the available charge is distributed across the parent peak and adduct(s). The current study systematically evaluated common liquid chromatography (LC) co...

In this letter we study the ground-state phase diagram of the one-dimensional Falicov-Kimball model. This model was proposed to describe metal-insulator transitions [ 1] and has since been investigated in connection with a variety of problems such as binary alloys [ 2], ordering in mixed-valence systems [ 3], and the formation of ionic crystals [ 4]. It is the latter language we shall use here,...

Homing endonucleases are site-specific DNA endonucleases that function as mobile genetic elements by introducing double-strand breaks or nicks at defined locations. Of the major families of homing endonucleases, the modular GIY-YIG endonucleases are least understood in terms of mechanism. The GIY-YIG homing endonuclease I-BmoI generates a double-strand break by sequential nicking reactions duri...

a binding study of nickel ions by a new recombinant human growth hormone (hgh), produced as an injected drug, has been done at 27˚c in nacl solution (50 mm) using an isothermal titration calorimetry. there is a set of three identical and non-interacting binding sites for nickel ions. the intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 40 μm and -16.5 kj/mol, re...

Electron paramagnetic resonance (EPR) spectroscopy was used to examine changes in internal structure and dynamics of the hammerhead ribozyme upon metal ion induced folding, changes in pH, and the presence and absence of ribozyme inhibitors. A nitroxide spin-label was attached to nucleotide U7 of the HH16 catalytic core, and this modified ribozyme was observed to retain catalytic activity. U7 wa...

the biosorption of heavy metals can be an effective process for the removal of such metal ions from aqueous solutions. in this study, the adsorption properties of nonliving biomass of phanerochaete chrysosporium for pb (ii) and zn (ii) were investigated by the use of batch adsorption techniques. the effects of initial metal ion concentration, initial ph, biosorbent concentration, stirring speed...

Whether electrons or ions are involved in triboelectric charging of insulators remains elusive. In keeping with the multidisciplinary nature of the problem, we designed polymers whose surface compositions (determined by XPS) differed from the bulk. Charging against insulators related to their topmost surface compositions, but against metals related to their bulk compositions. We propose the hyp...

In vivo biosensing requires stable transistor operation in high-salt concentration bodily fluids while exhibiting impermeability to mobile alkali ions that would otherwise render the metal-oxide-semiconductor (MOS) threshold voltage to drift. Metal oxide semiconductor capacitor structures using Al2O3 as the gate dielectric were soaked in a sterile physiological buffer solution (PBS) up to 24 ho...

A group of Cu,Zn-superoxide dismutases from pathogenic bacteria is characterized by histidine-rich N-terminal extensions that are in a highly exposed and mobile conformation. This feature allows these proteins to be readily purified in a single step by immobilized metal affinity chromatography. The Cu,Zn-superoxide dismutases from both Haemophilus ducreyi and Haemophilus parainfluenzae display ...

a proton nmr method for the drtermination of salvation numbers of alkaline earth cations with hexamethyl phosphoramide (hmpa) in nitromethane (nm) as diluents is described. the method is based on monitoring the resonance frequency of hmpa protons as a function of hmpa / metal ion molar ratio at constant metalion concentration. the average salvation number of cations,  , at any hmpa / metal ion ...