Objective(s):Eukaryotic translation initiation factor 4E (eIF4E) is overexpressed in cervical cancer (CC). However, the molecular mechanisms are unclear. This study aimed to investigate the molecular mechanism of eIF4E gene overexpression in CC. Materials andMethods:The human papillomavirus (HPV) type 18 E7 and eIF4E mRNAs were measured following knock down or overexpression of E7 gene by RT-P...

A rapid and reliable mating type assay for Fusarium circinatum was created by applying primers specific for the MAT1-1 and MAT1-2 mating type alleles to genomic DNA in a single PCR. A similar approach may be applied to fungi not previously shown to reproduce sexually, thus enabling studies of population structure and inheritance.

For the evaluation of autoantibodies in Pemphigus vulgaris (PV), an indirect ELISA assay was developed by using a semi-purified human epidermal skin extracted with approximately a molecular weight (M.W.) of 130-160 kDa. The evaluation of Pemphigus IgG, IgM and IgA autoantibodies in 75 patients and 50 control sera indicated that this indirect ELISA assay was a useful method for the detection of ...

In the present study, two cell-based systems for assessing Yang and Yin activities were first time used to investigate effect of ursolic acid (UA) oleanolic (OA). The results indicated that while UA was only active in assay, OA produced activity assay. Yang/Yin UA/OA may be attributed their distinct molecular structures, which confer differential ability interact with mitochondrial membrane or ...

background : gliomas are the most common primary brain tumors of the central nervous system. among a number of different bio-molecular events, molecular connections between oxidative stress pathways and their development is prevalent. oxidative stress is the consequence of an imbalance between pro-oxidant factors and antioxidant defense. this imbalance may lead to dna damage and changes in grow...