نتایج جستجو برای: mouse somatic cell nuclear transfer
تعداد نتایج: 2294906 فیلتر نتایج به سال:
Fertilized mouse zygotes can reprogram somatic cells to a pluripotent state. Human zygotes might therefore be useful for producing patient-derived pluripotent stem cells. However, logistical, legal and social considerations have limited the availability of human eggs for research. Here we show that a significant number of normal fertilized eggs (zygotes) can be obtained for reprogramming studie...
Studies on cell fusion-mediated nuclear reprogramming have led to the breakthrough of the induced pluripotent stem (iPS) cell technology. While this technology has neared stem cells to applications more than any other method, the mechanistic bases of reprogramming remain largely unsolved. In this context, comparative studies of oocyte and cell fusion-mediated reprogramming hold the greatest pro...
Nuclear transfer is a complex multistep procedure that includes oocyte maturation, cell cycle synchronization of donor cells, enucleation, cell fusion, oocyte activation and embryo culture. Therefore, many factors are believed to contribute to the success of embryo development following nuclear transfer. Numerous attempts to improve cloning efficiency have been conducted since the birth of the ...
BACKGROUND Over the past several years, mammals have been successfully cloned by either the splitting of an early stage embryo or nuclear transfer of adult somatic cells (NT) into oocytes. Although it has been 15 years since the generation of the first cloned mammals from somatic cells by NT, the success rate for producing live offspring by this technique is low regardless of the cell type and ...
After historic experiments by Briggs and King (Briggs and King, 1957; Briggs and King, 1952), animal cloning has emerged as a key approach to explore functional changes in the nuclear genome during differentiation (Gurdon, 1974; Gurdon, 1999; Kikyo and Wolffe, 2000; Rideout et al., 2001; Solter, 2000). Studies in amphibians have revealed that the potential of the nucleus to direct normal embryo...
Many of the structural and mechanistic requirements of oocyte-mediated nuclear reprogramming remain elusive. Previous accounts that transcriptional reprogramming of somatic nuclei in mouse zygotes may be complete in 24-36 hours, far more rapidly than in other reprogramming systems, raise the question of whether the mere exposure to the activated mouse ooplasm is sufficient to enact reprogrammin...
Dear Editor, Recent studies show that induced pluripo-tent stem cells (iPSCs) generated through ectopic expression of transcription factors retain an epigenetic memory of their original somatic cells (Kim et al., 2010; Polo et al., 2010) or aberrant silencing of a single imprinted gene cluster (Liu et al., 2010; Stadtfeld et al., 2010), which affects their developmental and differentiation pote...
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