نتایج جستجو برای: multiplex ligation dependentprobe amplification

تعداد نتایج: 97340  

Journal: :Frontiers in bioscience : a journal and virtual library 2007
Chelsee Ann Hewitt Catherine Louise Carmichael Ella Jane Wilkins Ping Zhi Fang Cannon Melanie April Pritchard Hamish Steele Scott

The Ts65Dn mouse is the most widely investigated segmentally trisomic mouse model of Down syndrome. Quantitative PCR based methods are the preferred way of detecting the trisomic segment for genotyping purposes. However, identification of a 1.5 fold difference in target DNA is at the limit of detection of most quantitative PCR based methods, and in practice this can lead to difficulties in assi...

Journal: :Haematologica 2008
Paola Quarello Emanuela Garelli Alfredo Brusco Adriana Carando Patrizia Pappi Marco Barberis Valentina Coletti Maria Francesca Campagnoli Irma Dianzani Ugo Ramenghi

2015
Dale Muzzey Eric A. Evans Caroline Lieber

Identifying disease-causing mutations in DNA has long been the goal of genetic medicine. In the last decade, the toolkit for discovering DNA variants has undergone rapid evolution: mutations that were historically discovered by analog approaches like Sanger sequencing and multiplex ligation-dependent probe amplification ("MLPA") can now be decoded from a digital signal with next-generation sequ...

2007
A De Luca V Lanari L Bernardini L Divona S Giustini L Sinibaldi A Novelli I Torrente A Schirinzi B Dallapiccola

A De Luca, I Bottillo, M C Dasdia, A Morella, V Lanari, L Bernardini, L Divona, S Giustini, L Sinibaldi, A Novelli, I Torrente, A Schirinzi, B Dallapiccola . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....

Journal: :iranian biomedical journal 0
kimia ghaffari mehrdad hashemi elmira ebrahimi reza shirkoohi

background: baculoviral inhibitor of apoptosis repeat-containing 5 (birc5) gene is an inhibitor of apoptosis that expresses in human embryonic tissues but it is absent in most healthy adult tissues. the copy number of birc5 has been indicated to be highly increased in tumor tissues; however, its association with the age of onset in breast cancer is not well understood. methods: forty tumor tiss...

Journal: :Journal of microbiological methods 2010
Alina Deshpande Jason Gans Steven W Graves Lance Green Laura Taylor Heung Bok Kim Yuliya A Kunde Pascale M Leonard Po-E Li Jacob Mark Jian Song Momchilo Vuyisich P Scott White

We have developed a rapid (under 4 hours), multiplex, nucleic acid assay, adapted to a microsphere array detection platform. We call this assay multiplex oligonucleotide ligation-PCR (MOL-PCR). Unlike other ligation-based assays that require multiple steps, our protocol consists of a single tube reaction, followed by hybridization to a Luminex microsphere array for detection. We demonstrate the...

ژورنال: :genetics in the 3rd millennium 0
بهروز ابراهیمی behrouz ebrahimzadeh یوسف شفقتی yousef shafeghati نوید المدنی navid almadani روشنک وامقی roshank vameghi فیروزه ساجدی firouzeh sajedi ساغر قاسمی فیروز آبادی saghar ghasemi firouzabadi حسین نجم آبادی

سندرم آنجلمن یک اختلال ژنتیکی پیچیده است که سیستم عصب مرکزی را گرفتار می کند . اغلب موارد سندرم آنجلمن (حدود 70%) زمانی رخ می دهد که یک قطعه از کروموزوم 15 (15q11-q13)مادری حذف میشود . علایم اصلی این سندرم شامل تاخیر تکامل ، ناتوانی ذهنی، اختلال کلامی شدید و مشکلات حرکتی و تعادلی ( آتاکسیا) میباشد . در این گزارش یک پسر بچه 8 ساله با علایم ناتوانی ذهنی ، تشنج ، خنده اضافی و اختلال حرکتی در دستها...

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