background escherichia coli o157:h7, vibrio cholerae, and salmonella typhimurium are pathogenic bacteria found in contaminated water and food. no assay method is currently available on simultaneous detection or identification of all the three pathogens. our aim was to develop a rapid and reliable method for this purpose. materials and methods a protocol for sample collection, and a pcr procedur...

conclusions this is the first report on the prevalence of iron acquisition genes among a. baumannii isolates collected from patients with urinary tract infections. the high prevalence of iron acquisition genes in a. baumannii isolates suggests that these virulence factors play an important role in the development of urinary tract infections. results a high prevalence of genes encoding iron acqu...

OBJECTIVE To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. METHODS Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance t...

A rapid method for detection of Escherichia coli O157: H7 using multiplex PCR was developed. Two oligonucleotide primer pairs were used for simultaneously detection of vt encoding verotoxin genes for virulence factor and rfbO157 encoding the O-antigen specific for E. coli O157: H7. Multiplex PCR generated two products of 215 bp and 420 bp for vt and rfbO157, respectively. Multiplex PCR detected...

coagulase-positive and coagulase negative, methicillin-resistant staphylococci are major causes of serious nosocomial infections and it is very important to have a reliable test to detect these bacteria. a multiplex polymerase chain reaction (mpcr) was used on 100 clinical samples for simultaneous amplification of the universal bacterial, mec-a encoding the penicillin binding protein 2a, which ...

OBJECTIVE To determine the incidence of Mycobacterium tuberculosis complex and non-tuberculous mycobacterial isolates in the routine setting of a large general hospital using an "in-house" multiplex polymerase chain reaction method and to establish a paradigm for the definitive identification of mycobacteria isolated using semi-automated equipment. METHODS Established tests, including polymer...