نتایج جستجو برای: mutant

تعداد نتایج: 129581  

Abbas Tahzibi, Fatemeh Kamal, Mahnaz Mazaheri Assadi,

A Pseudomonas aeruginosa mutant derived by random mutagenesis with N-methyl-N¢-nitro-N-nitrosoguanidine, producing high levels of the rhamnolipid biosurfactants was selected on Siegmund-Wagner (SW) plates. The mutant designated P. aeruginosa PTCC1637 produces rhamnolipids at concentrations 10 time more than parent strain. NMR analysis and surface tension measurement showed that the biosurfactan...

In order to assess the responses of Hashemi rice genotype and its advanced mutant line under salinity stress of 100 mM Sodium chloride (NaCl) for three and six days the shoot samples were taken for biochemical analysis. This experiment was performed in split plot based on randomized complete block design with three replications. The main factor was factorial combination of saline treatmen...

A one kb portion of the rpoS gene from Flexibacter chinensis was isolated by PCR, sequenced and compared to the rpoS gene of a variety of other organisms. The gene was found to be 98% similar to previously sequenced genes. Mutation of the rpoS gene with tri-parental mating produced strain JR101 and the growth rate of the mutant was compared with that of the wild-type. The mutant grew slower, an...

DREB transcription factors are one of the largest families of transcription factors in plants that are believed to play important regulatory functions in tolerance to environmental stress, but there is no complete information on their role in controlling the morphological and antioxidant properties of developed plants under normal conditions. In this study, some phenotypic characteristics of th...

ژورنال: Medical Laboratory Journal 2016
Ahani Azari , Ania, Nayeri Fasaei , Bahar, Zahraei Salehi , Taghi,

ABSTRACT         Background and Objective: In this study, we compared the susceptibility of Salmonella typhimurium phoP mutant and its parent to stress conditions that the pathogen may encounter in a host.         Methods: For this purpose, we used the phoP deletion mutant constructed in our previous study. In order t...

Fatemeh Kamal, Hadi Mehrgan, Mahnaz Mazaheri Assadi, Seyed Alireza Mortazavi,

Xanthan gum is a microbial polysaccharide of great commercial importance as it has unusual rheological properties in solution and consequent range of applications. In this study, a series of mutants were isolated from Xanthomonas campestris PTCC 1473 by ethyl methanesulfonate mutagenesis. The polysaccharide yield of one mutant, XC1473E2, was 30% better than that of the parent strain. It also sh...

Journal: :jundishapur journal of microbiology 0
farshad darvishi department of microbiology, university of maragheh, maragheh, ir iran; department of microbiology, faculty of science, university of maragheh, p.o. box 55181-83111, maragheh, ir iran. tel: +98-4212276068, fax: +98-4212276060

conclusions results of this study could be used to find suitable targets for inverse metabolic engineering and site-directed mutagenesis to achieve stable and high-level extracellular lipase production in y. lipolytica and other yeasts. results two nucleotide substitutions were found in the extracellular lipase lip2 orfs of y. lipolytica u6 mutant strain compared to the wild-type strain. in thi...

Journal: :journal of medical bacteriology 0
mohammadsadegh madadi department of clinical sciences, faculty of veterinary medicine, university of tabriz, tabriz, ir iran. sara mirzaie department of animal, poultry and aquatic sciences, institute for agricultural research, iranian organization for science and technology, tehran, ir iran. mohammad hassanzadeh department of poultry diseases, faculty of veterinary medicine, university of tehran , ir iran.

background : salmonella actively stimulates its own uptake into the epithelial cells by inducing cytoskeleton rearrangements and membrane ruffling triggered by some proteins secreted by salmonella into the cytosol of the epithelial cells via a type iii secretion system (ttss) encoded by genes of the salmonella pathogenicity island 1 (spi-1). hila is a transcriptional activator encoded on salmon...

Journal: :journal of crop protection 2015
mojdeh akandeh farhan kocheili mahmoud soufbaf arash rasekh

reproductive parameters and ovipositional preference of plutella xylostella were assessed on some mutant genotypes of canola (rgs 8-1, rgs 10-2, rgs 8-13, zar 9-9 and talaye 8-3) and their cultivars (rgs, zar, talaye) under greenhouse condition (21 ± 6 °c, 65 ± 10 rh and a photoperiod of 16l: 8d h). comparison of the reproductive parameters on rgs and its mutant genotypes revealed that mutant g...

Journal: :iranian biomedical journal 0
بیتا بخشی bita bakhshi مینا بوستان شناس mina boustanshenas مسعود قربانی masoud ghorbani

background: cholera toxin b subunit (ctb) has been extensively considered as an immunogenic and adjuvant protein, but its yield of expression is not satisfactory in many studies. the aim of this study was to compare the expression of native and mutant recombinant ctb (rctb) in pqe vector. methods: ctxb fragment from vibrio cholerae o1 atcc14035 containing the substitution of mutant ctxb for ami...

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