نتایج جستجو برای: mutant strains
تعداد نتایج: 270616 فیلتر نتایج به سال:
abstract in this study a greenhouse experiment was conducted to evaluate the role of acc deaminase enzyme on alleviation of salinity stress of canola plant. canola plants were exposed to pseudomonas fluorescens strain p12 which contains a rhizobacter capable of producing pseudomonas fluorescens in comparison with plants exposed to mutant pm12 unable to produce the required enzyme. the mutant wa...
Hemochromatosis is caused by mutations in HFE, a protein that competes with transferrin (TF) for binding to transferrin receptor 1 (TFR1). We developed mutant mouse strains to gain insight into the role of the Hfe/Tfr1 complex in regulating iron homeostasis. We introduced mutations into a ubiquitously expressed Tfr1 transgene or the endogenous Tfr1 locus to promote or prevent the Hfe/Tfr1 inter...
For most imprinted genes, a difference in expression between the maternal and paternal alleles is associated with a corresponding difference in DNA methylation that is localized to a differentially methylated domain (DMD). Removal of a gene's DMD leads to a loss of imprinting. These observations suggest that DMDs have a determinative role in genomic imprinting. To examine this possibility, we i...
Recent studies have shown that the ubiquitin proteasome pathway (UPP) is closely related to immune defense. We have identified a ubiquitin-conjugating enzyme E2 from the Chinese white shrimp Fenneropenaeus chinensis (FcUbc). Injection of recombinant FcUbc protein (rFcUbc) reduced the mortality of shrimp infected with White Spot Syndrome Virus (WSSV) and inhibited replication of WSSV. rFcUbc, bu...
To study HIV-1 escape from a coreceptor antagonist, the R5 primary isolate CC1/85 was passaged in peripheral blood mononuclear cells with increasing concentrations of the CCR5-specific small molecule inhibitor, AD101. By 19 passages, an escape mutant emerged with a >20,000-fold resistance to AD101. This virus was cross-resistant to a related inhibitor, SCH-C, and partially resistant to RANTES b...
We report the characterisation of 27 cardiovascular-related traits in 23 inbred mouse strains. Mice were phenotyped either in response to chronic administration of a single dose of the beta-adrenergic receptor blocker atenolol or under a low and a high dose of the beta-agonist isoproterenol and compared to baseline condition. The robustness of our data is supported by high trait heritabilities ...
Human mucopolysaccharidosis VII (MPS VII, Sly syndrome) results from a deficiency of beta-glucuronidase (GUS) and has been associated with a wide range in severity of clinical manifestations. To study missense mutant models of murine MPS VII with phenotypes of varying severity, we used targeted mutagenesis to produce E536A and E536Q, corresponding to active-site nucleophile replacements E540A a...
Underexpression of the transcriptional coactivator PGC-1 is causally linked to certain neurodegenerative disorders, including Huntington’s Disease (HD). HD pathoprogression is also associated with aberrant NMDAR activity, in particular an imbalance between synaptic versus extrasynaptic (NMDAR ) activity. Here we show that PGC-1 controls NMDAR EX activity in neurons and that its suppression cont...
conclusions results of this study could be used to find suitable targets for inverse metabolic engineering and site-directed mutagenesis to achieve stable and high-level extracellular lipase production in y. lipolytica and other yeasts. results two nucleotide substitutions were found in the extracellular lipase lip2 orfs of y. lipolytica u6 mutant strain compared to the wild-type strain. in thi...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید