A serological study of twenty three European bison (Bison bonasus) derived from Northern-East Poland for the seroprevalence of Mycobacterium avium subsp. paratuberculosis, Mycoplasma bovis, Mycoplasma mycoides subsp. mycoides SC, Mycoplasma agalactiae and Mycoplasma capricolum subsp. capripneumoniae was conducted. Only specific antibodies to M. bovis were detected in two animals (8.7%) which we...

AIMS To develop an immunomagnetic capture (IMC) to detect viable Mycoplasma agalactiae in routine ovine milk samples. METHODS AND RESULTS Polyclonal antibodies against two M. agalactiae membrane surface proteins (P80 and P55) were covalently conjugated to magnetic beads (MBs) to form MB-Ab80 and MB-Ab55. Mycoplasma agalactiae cells were captured by a specific antigen-antibody reaction and mag...

An immunodominant protein, P40, of Mycoplasma agalactiae was analyzed genetically and functionally. The gene encoding P40 was cloned from type strain PG2, sequenced, submitted to point mutagenesis in order to convert mycoplasma-specific TGA(Trp) codon to the universal TGG(Trp) codon, and subsequently expressed in Escherichia coli. Nucleotide sequence-derived amino acid sequence comparisons reve...

El estudio tuvo como objetivo determinar la prevalencia de Toxoplasma gondii y Mycoplasma agalactiae en ovinos caprinos del sur La Guajira norte Cesar parte establecimiento un perfil serológico estas especies animales región colombiana. Las muestras suero (n=1039) fueron analizadas con kits comerciales ELISA específicos para estos dos patógenos. global encontrada fue 25.1 18.3% T. 0.12 0.8% M. ...

A major surface antigenic lipoprotein of Mycoplasma agalactiae, promptly recognized by the host's immune system, was characterized. The mature product, P48, showed significant similarity and shared conserved amino acid motifs with lipoproteins or predicted lipoproteins from Mycoplasma fermentans, Mycoplasma hyorhinis, relapsing fever Borrelia spp., Bacillus subtilis, and Treponema pallidum.

Contagious agalactiae is a disease of sheep & goats, which is caused by Mycoplasma agalactiae and induces heavy economic losses. The aim of this study was to isolate and identify M. agalactia from sheep and goats at Fars province. Clinical samples such as milk, eye swabs and synovial fluids were collected from infected animals. The collected samples were transferred in a transport medium which ...

Abortion is one of the most important economic issues in sheep flocks. Chlamydophila abortus is an agent of enzootic abortions in sheep. Mycoplasma agalactiae is the main etiological agent of contagious agalactia, which can cause abortion in sheep. The aim of this study was to investigate the prevalence of M. agalactiae and C. abortus among aborted ovine fetu...

Mycoplasma agalactiae isolates from Spain were genetically characterized to investigate their genomic diversity and to better understand their relationship to isolates from other countries. Molecular typing revealed a high genomic homogeneity in Spanish M. agalactiae isolates, which clearly shows the circulation of one endemic clonal population.

We evaluated the capacity of the Mycoplasma agalactiae p40 gene as a diagnostic marker for contagious agalactia in sheep by quantitative real-time PCR. The p40 gene encodes an immunodominant adhesin that plays a key role in cytoadhesion of M. agalactiae. The assay was 100% specific, with an analytical sensitivity of 1 genome equivalent (GE), a quantification that is highly linear (R(2) > 0.992)...

contagious agalactiae (c.a.) is one of the most common disease affecting small ruminants which is caused by mycoplasma agalactiae. this disease is particularly widespread around the world and iran is one of the countries that c.a. is present. the aim of this study was isolation and identification of m. agalactiae (mg) with culture and pcr technique in milk samples in kordestan province, iran. a...