Aberrant DNA methylation has been reported as an important phenotype in acute myeloid leukemia. However the clinical significance of methylation changes has not been clear yet. In this study methylation Specific Melting Curve Analysis (MS-MCA) and real time PCR was used to assess the CDKN2B promoter hyper-methylation and gene expression in 59 Iranian acute myeloid leukemia (AML) patients. The i...

Background Placentation shares many analogues with the development of tumors such as rapid proliferation, invasiveness, gene expression profiles especially the expression of tumor suppressor genes, oncogenes and matrixmetallo proteinases (MMPs). Thus, a placenta has been described as a pseudomalignant tissue. However, placentation is tightly regulated and any deregulation of this pseudomalignan...

methylated and un-methylated control dna is an important part of dna methylation studies. although human and mouse dna methylation control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. in this paper a simple method of generating methylated and un-methylated control dna is described. whole...

The dual reactivity of N,N,N-trimethylanilinium salts towards arylation and methylation is decoupled in this mechanistic investigation to enable more strategic application these either reaction class.

background : ovarian cancer is the leading cause of death among gynecological cancers. changes in the methylation of brca1 and brca2 may be an effective mechanism for breast and ovarian cancer. this study evaluates the protein expression and methylation status of brca2 in iranian patients. methods : we assessed 60 mullerian-type ovarian cancers by methylation-specific pcr assays and immunohisto...

dna methylation as epigenetic mark plays a key role in normal differential and developmental processes as well as in dynamic gene regulation at the genomic level. to assess dna methylation pattern in different developmental stages of aeluropus littoralis, methylation sensitive amplified polymorphism (msap) was used. methylation and demethylation status at the ccgg recognition site were tracked ...

Promoter methylation is one of the main epigenetic mechanisms that lead to the inactivation of tumor suppressor genes during carcinogenesis. Due to the reversible nature of DNA methylation, many studies have been performed to correct theses epigenetic defects by inhibiting DNA methyltransferases (DNMTs). In this case novel therapeutics especially siRNA oligonucleotides have been used to specifi...

Methylated and un-methylated control DNA is an important part of DNA methylation studies. Although human and mouse DNA methylation control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. In this paper a simple method of generating methylated and un-methylated control DNA is described. Whole...