Human metapneumovirus (hMPV) is an etiologic agent of respiratory tract infections. In this study, we compared the sensitivity and specificity of real-time reverse transcription (RT)-polymerase chain reaction (PCR), conventional RT-PCR, and nested PCR in detecting hMPV genes. A total of 146 clinical specimens from 143 patients who showed acute respiratory tract infection symptoms were tested by...

BACKGROUND AFRIMS longitudinal dengue surveillance in Thailand depends on the nested RT-PCR and the dengue IgM/IgG ELISA. OBJECTIVE To examine and improve the sensitivity of the nested RT-PCR using a panel of archived samples collected during dengue surveillance. STUDY DESIGN A retrospective analysis of 16,454 dengue IgM/IgG ELISA positive cases collected between 2000 and 2013 was done to i...

bovine viral diarrhoea virus (bvdv) is an important pathogen of dairy cattle. in this study, bulk milk samples representing a total of 4105 milking cows, from 18 dairy cattle herds in the suburb of mashhad- iran, were tested for presence of bvdv by the use of a nested reverse transcription polymerase chain reaction (nested rt- pcr) assay. non of the cows in the herds had been vaccinated against...

Serum samples from 96 Spanish hemodialysis patients, as well as serial dilutions of RNA extracted from a reference strain of hepatitis G virus (HGV), were tested for HGV or GB virus C (GBV-C) RNA. Two different reverse transcription (RT)-PCR-based methods of detection were compared for the ability to detect RNA extracted from the samples: an RT-nested PCR assay with primers derived from the 5' ...

A traditional nested reverse transcription-polymerase chain reaction (RT-PCR) assay specific for eastern equine encephalomyelitis (EEE) virus was designed to multiplex with a previously described West Nile (WN) virus nested RT-PCR assay. Differentiation of EEE and WN was based on base pair size of the amplified product. One hundred fifty-seven mammalian and avian brain tissues were tested by EE...

background: hepatitis g virus (gbv-c) is a single strand rnapositive virus and is a member of flaviviridae family. infection with the virus is prevalent add has worldwide distribution. accurate diagnosis of the virus is very important, because of co-infection with other important viruses like hcv, hbv and hiv, it may influence on pathogenesis, disease progression and response to viral therapy. ...