Acacia aroma, native plant from San Luis, Argentina, is commonly used as antiseptic and for healing of wounds. The present study was conducted to investigate the in vitro cytotoxicity and genotoxicity of hot aqueous extract (HAE) and ethanolic extract (EE) of A. aroma. The cytotoxic activity was assayed by neutral red uptake assay on Vero cell. Cell treatment with a range from 100 to 5000 μg/mL...

conclusions genome instability in addicted patients was demonstrated in this study. controversially, considering incoherent results of previous studies and our data, more studies in longer duration of methadone use are needed to elucidate the consequence. results the mutagenicity index in addicted group was robustly higher than healthy volunteers. fortunately, this significant difference was lo...

background the increasing use of herbal drugs and their easy availability have necessitated the use of mutagenicity tests to analyze their toxicity and safety. objectives the aim of this study was to determine the in vitro mutagenicity of artemisia draconculus l., a herbal drug, by performing single cell gel electrophoresis (scge). materials and methods in this study, we obtained a herbal drug ...

Abstract The possible effects of wastewater treatment products on mussels (Mytilus galloprovincialis) were studied. Three common pollution biomarkers used: neutral red retention assay, malondialdehyde contents and DNA damage through comet assay. Two groups treated in the laboratory for 25 days with 20 40 %v/v collected after chlorination, control formed a third group. results showed statistical...

Apoptosis is a form of programmed cell death that has only recently been implicated in the pathogenesis of cardiac diseases, including ischemia and reperfusion damage and in response to cytokines and other apoptotic triggers. Methods to study apoptosis range from measures of early changes in morphology to late events such as DNA laddering. In studying apoptosis in the heart, the application of ...

The alkylating mutagens N-methyl-N-nitrosourea (MNU) and methyl methanesulfonate (MMS) were studied for their potential to induce DNA strand breaks and abasic (AP) sites in meristematic nuclei of Vicia faba root tips by the comet assay. The alkaline unwinding/neutral electrophoresis (A/N) and alkaline unwinding/alkaline electrophoresis (A/A) protocols were used for detection of DNA damage. With...

Reactive species such as free radicals are constantly generated in vivo and DNA is the most important target of oxidative stress. Oxidative DNA damage is used as a predictive biomarker to monitor the risk of development of many diseases. The comet assay is widely used for measuring oxidative DNA damage at a single cell level. The analysis of comet assay output images, however, poses considerabl...

Phthalates are industrial chemicals widely used in many commercial applications. The general population is exposed to phthalates through consumer products as well as through diet and medical treatments. To determine whether environmental levels of phthalates are associated with altered DNA integrity in human sperm, we selected a population without identified sources of exposure to phthalates. O...

background: alpinia galanga l. belonging to the family zingiberaceae is widely grown in the state of kerala, india. they are effective antioxidant and free radical scavenger under both in vitro and in vivo condition. the efficacy of the isolated flavonoids in conferring protection from radiation induced damages to genomic dna was studied. materials and methods: the bioflavonoids, galangin and k...

Introduction: Infectious diseases are one of the main causes of death worldwide. This has driven scientists to invest in extraction and identification of antimicrobial agents from natural toxins and presentation of novel antibiotics and vaccines. The aim of the current study is to investigate the antibacterial and cytotoxicity effects of the protective antigen domain 4 (PAD4) from Bacillus anth...