The sulfate-reducing bioprocess is a promising technology for the treatment of heavy metal-containing wastewater. This work was conducted to investigate the possibility of promoting heavy metal removal by the addition of citrate to mask Ni2+ toxicity to sulfate-reducing bacteria (SRB) in batch reactors. SRB growth was completely inhibited in Ni2+-containing medium (1 mM) when lactate served as ...

Bacterial chemotaxis is of medical, biological, and geological significance. Despite its importance, current chemotaxis measurements fail to account for the speciation of the chemical effector and the protonation state of the bacterial surface. We hypothesize that adsorption of Ni2+ onto the surface of Escherichia coli can influence its effective concentration and therefore influence its abilit...

The chelating agents EDTA, o-phenanthroline, nitrilotriacetic acid (NTA), ethylenediamine-bis(o-hydroxyphenylacetic acid) (EDDA) or dimethylglyoxime prevented the expression of hydrogenase activity in batch cultures of nitrogen-fixing Azotobacter chroococcum, but did not inhibit preformed enzyme. The inhibition was reversed either by adding a mixture of trace elements (Cu2+, Mn2+, Zn2+, Co2+) o...

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is a universally used method for determining approximate molecular weight (MW) in protein research. Migration of protein that does not correlate with formula MW, termed "gel shifting" appears to be common for histidine-rich proteins but not yet studied in detail. We investigated "gel shifting" in Ni2+-binding histidine-rich H...

The adsorption characteristics and mechanisms of Ni2+ on four-standard biochars produced from wheat straw pellets (WSP550, WSP700) and rice husk (RH550, RH700) at 550 and 700 °C, respectively, were investigated. The kinetic results show that the adsorption of Ni2+ on the biochars reached an equilibrium within 5 min. The increase of the solid to liquid ratio resulted in an increase of Ni2+ remov...

The difficulty of rapid, definitive diagnosis of myocardial ischemia leads to unnecessary hospital admissions and treatment delays. Previously, decreased metal binding affinity in human serum was investigated as a marker for myocardial ischemia. Polymerized Crystalline Colloidal Array (PCCA) sensors for Ni2+ may be useful in developing a point-of-care test to determine metal binding affinity in...

The magnetic properties and structural phase transformations of Heusler alloys Ni2+ Mn1 Ga and Ni2 Mn1+ 2Ga1+ 2 were investigated. It was found that the martensitic transformation temperature increases and the Curie temperature decreases with decreasing Mn concentration in the Ni2+ Mn1 Ga system, but the magnetic properties decrease faster than those do in the Ni2 Mn1+ 2Ga1+ 2 system. It sugges...

Ethanol is an alternative fuel for direct alcohol fuel cells, in which the electrode materials are commonly based on Pt or Pd. Owing to the excellent promotion effect of Ni2 P that was found in methanol oxidation, we extended the catalyst system of Pt or Pd modified by Ni2 P in direct ethanol fuel cells. The Ni2 P-promoted catalysts were compared to commercial catalysts as well as to reference ...

The epithelial Na+ channel (ENaC) is modulated by various extracellular factors, including Na+, organic or inorganic cations, and serine proteases. To identify the effect of the divalent Ni2+ cation on ENaCs, we compared the Na+ permeability and amiloride kinetics of Xenopus ENaCs (xENaCs) and rat ENaCs (rENaCs) heterologously expressed in Xenopus oocytes. We found that the channel cloned from ...

Department of Chemistry, Texas A & M Un E-mail: [email protected] Department of Chemistry, Tamkang Univer † Electronic supplementary informati spectroscopic, additional electrochemic crystallographic data (CIF) from the s [Ni2–Fe2] , [Ni2–Fe] , and computation crystallographic data for the complexes [ deposited in the Cambridge Crystallog (CCDC 1045461), [Ni2–Fe2] 2+ (CCDC 104 For ESI and ...