The best way to control mosquito borne diseases is to control the mosquito population. The present study was executed to evaluate the mosquito larvicidal activity of Nicotiana plumbaginifolia leaves against malarial vector Anopheles stephensi.Crude extracts and three different solvent extracts were bio-assayed against An. stephensi larvae. LC50 and LC90 values were determined appropriately. Qua...

The sensitivity of the Nicotiana plumbaginifolia wild-type and phytochrome B (hlg) mutant to photoperiod was investigated. Leaf number at bolting and rosette-leaf movement were measured under conditions of different light quality and photoperiod. Bolting in wild-type plants was slightly delayed under short days (SD), compared to plants grown under long days (LD). The hlg mutant bolted later tha...

Nicotiana plumbaginifolia protoplasts were directly transformed by PEG treatment with a cloned 16S rRNA gene isolated from a double antibiotic-resistant Nicotiana tabacum plastid mutant. Putative plastid transformants were selected in cell culture by their spectinomycin resistance and identified by their unselected streptomycin resistance. Alternatively, cell lines were selected in the presence...

Although S-locus RNases (S-RNases) determine the specificity of pollen rejection in self-incompatible (SI) solanaceous plants, they alone are not sufficient to cause S-allele-specific pollen rejection. To identify non-S-RNase sequences that are required for pollen rejection, a Nicotiana alata cDNA library was screened by differential hybridization. One clone, designated HT, hybridized strongly ...

The Nicotiana plumbaginifolia gnl gene encoding a 8-1,3-glucanase isoform has been characterired. The gnl product represents an isoform distinct from the previously identified tobacco 8-1,3-glucanases. By expressing gnl in Escherichia coli, we have determined directly that the encoded protein does, indeed, correspond to a &1,3glucanase. In N. plumbaginifolia, gnl was found to be expressed in ro...