The present study was aimed at the development of a somoclonal variant and isozyme marker for Phyllanthus amarus Schum & Thonn using inter-nodal segments and the enzyme peroxidase. Maximum callus proliferation was obtained on Murashige and Skoog’s medium supplemented (MS) with 1.0 mg/l of 2,4-Dichlorophenoxyacetic acid. Three weeks-old pale yellowish white semi-friable callus was used for organ...

the present study was aimed at the development of a somoclonal variant and isozyme marker for phyllanthus amarus schum & thonn using inter-nodal segments and the enzyme peroxidase. maximum callus proliferation was obtained on murashige and skoog’s medium supplemented (ms) with 1.0 mg/l of 2,4-dichlorophenoxyacetic acid. three weeks-old pale yellowish white semi-friable callus was used for o...

In vitro propagation of rose has played a very important role in rapid multiplication of species with desirable traits and production of healthy and disease-free plants. Micropropagation using nodal segments of Rosa canina under different combinations of BAP, GA3 and NAA on MS and VS, medium was investigated. The results showed that the highest shoot proliferation was obtained on VS medium cont...

Andrographis lineata Nees (Acanthaceae) is an endemic herb rich in flavonoids and diterpenoids which have pharmaceutical importance. Seedling explants source has not been studied for micropropagation and hence the present investigation has been taken up. An efficient protocol was developed for aseptic seed germination and in vitro shoot multiplication from different aseptic seedling explants. 3...

An efficient micropropagation protocol of Bacopa monnieri (L.) Pennell through two-stage culture nodal segments and ex vitro acclimatizationShashikanta Behera, Nibedita Nayak, Shasmita, Durga P. Barik, Soumendra K. Naik

Multiple shoots were obtained from nodal segments of mature trees of Elaeagnus angustifolia L. cultured on MS medium (Murashige and Skoog 1962) supplemented with 0, 0.88 or 2.22 micro M N(6)-benzyladenine. When nodal segments taken from the in vitro proliferated shoots were cultured under the same conditions, additional multiple shoots were obtained. Rooting of the in vitro propagated shoots wa...

in vitro propagation of rose has played a very important role in rapid multiplication of species with desirable traits and production of healthy and disease-free plants. micropropagation using nodal segments of rosa canina under different combinations of bap, ga3 and naa on ms and vs, medium was investigated. the results showed that the highest shoot proliferation was obtained on vs medium cont...