We describe a relatively rapid radiochemical assay for determining plasma non-esterified fatty acids, by use of a Dole-type (J. Clin. Invest. 35: 150, 1956) extraction and formation of 57Co soaps. Results correlated well (r = 0.957) with those by gas-liquid chromatography. The between-batch CV for 1.12 mmol of non-esterified fatty acids per liter was 7%; the sensitivity is estimated to be 22 mu...

abstract         background and objective: the relationship between diabetes mellitus and increased risk of cardiovascular diseases has been demonstrated. the aim of this study was to determine the fatty acid profile of epicardial adipose tissue in diabetic and non-diabetic patients with cardiovascular disease.          methods: in this study, 28 diabetic and 40 nondiabetic patients were evalua...

Dyslipidaemia, with elevations of circulating triacylglycerols (triglycerides) and non-esterified (free) fatty acids, and hyperinsulinaemia are often found in the same subjects, the so-called 'insulin resistance syndrome'. The present study aims to investigate how elevated levels of non-esterified fatty acids, hyperinsulinaemia and the combination of these factors affects endothelium-dependent ...

Atherosclerosis is a major complication of diabetes, yet the reason for this remains obscure. Mechanisms of plaque formation are discussed and, in particular, metabolic alterations in the postprandial phase in diabetes are examined. A major metabolic effect of insulin deficiency is a failure to suppress non-esterified fatty acids. The importance of non-esterified fatty acids in the formation of...

Although many studies have been conducted on the components present in human breast milk (HM), research on the differences of chemical metabolites between HM, bovine milk (BM) and formula milk (FM) is limited. This study was to explore the chemical diversity of HM, BM and FM by metabolomic approaches. GC-TOFMS and UPLC-QTOFMS were applied to investigate the metabolic compositions in 30 HM sampl...

Abbreviations: EC – embryogenic calli; EFA – esterified fatty acids; FA – fatty acids; FAME – fatty acid methyl ester; FFA – free fatty acid; GC – gas chromatography; GC-MS – gas chromatography and mass spectrometry; HS – hypocotyl segments; HSC – hypocotyl segments with incipient calli formation; LCFA – long-chain fatty acid; MCFA – mediumchain fatty acids; NEC – non-embryogenic calli; PCA – p...