this study included 81 samples which belonged to 13 speices from myriophyllum genus. both nrdna its1 and its2 and cpdna matk, rbcl and trnh-psba loci were used for diagnose of invasive from native plant. the nrdna its1and its2 data proved highly variable and could differentiate between all but had low pcr amplification success,based on these result they would not recommend as a suitable barcode...

Ganoderma has been considered a very difficult genus among the polypores to classify and is currently in a state of taxonomic chaos. In a study of Ganoderma collections including numerous type specimens, we found that six species namely G. cupreum, G. densizonatum, G. limushanense, G. mastoporum, G. orbiforme, G. subtornatum, and records of G. fornicatum from Mainland China and Taiwan are very ...

With the decreasing cost and availability of many newly developed bioinformatics pipelines, next-generation sequencing (NGS) has revolutionized plant systematics in recent years. Genome skimming been widely used to obtain high-copy fractions genomes, including plastomes, mitochondrial DNA (mtDNA), nuclear ribosomal (nrDNA). In this study, through simulations, we evaluated optimal (minimum) dept...

This work aims to analyze the phylogeography of Schizolobium parahyba(Fabaceae), which includes two varieties with a disjunct distribution, from Southern Brazil to Central America. Neotropical rain forests, focus on four largest wet forests: Atlantic forest, Amazonian forests, Andean forest and Central America forest. The genetic diversity and differentiation of populations among S. parahyba po...

Dried parts of different plant species often look alike, especially in powdered form, making them very difficult to identify. Ruta graveolens, sold as a dried medicinal herb, can be adulterated with Euphorbia dracunculoides. The genomic DNA was isolated from the leaf powder (100 mg each) using the modified CTAB method. Internal transcribed spacer sequences of nuclear ribosomal DNA (nrDNA-I...

A previous study using data from nrDNA (ITS), maldehy, and petN-psbM (cpDNA) confirmed that allopatric hybridization is occurring at Wallowa, OR, eastern WA, and southeastern BC into western Montana. nrDNA was found to be of less use in detecting hybrids than a single copy nuclear gene (SCN), maldehy. This might to be due to concerted evolution in nrDNA or relictual effects from ancient speciat...

Polyploidy is an important evolutionary process in plants, but much remains to be learned about the evolution of gene expression in polyploids. Evolution and expression of the 18S-5.8S-26S ribosomal gene family was investigated at homeologous loci in the Glycine subgenus Glycine perennial soybean polyploid complex, which consists of several diploid genomes that have formed allopolyploids in var...

The taxonomy and phylogeny of Asian Meconopsis (Himalayan blue poppy) remain largely unresolved. We used the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) and the chloroplast DNA (cpDNA) trnL-F region for phylogenetic reconstruction of Meconopsis and its close relatives Papaver, Roemeria, and Stylomecon. We identified five main clades, which were well-supported in th...

Whole genome sequencing is helping generate robust phylogenetic hypotheses for a range of taxonomic groups that were previously recalcitrant to classical molecular phylogenetic approaches. As a case study, we performed a shallow shotgun sequencing of eight species in the tropical tree family Chrysobalanaceae to retrieve large fragments of high-copy number DNA regions and test the potential of t...

Coniothyrium-like fungi are common wood and soil inhabitants and hyperparasites on other fungi. They belong to different fungal genera within the Pleosporales. Several isolates were obtained on wood of different Prunus species (plum, peach and nectarine) from South Africa, on Actinidia species from Italy and on Laurus nobilis from Turkey. Morphological and cultural characteristics as well as DN...