Two novel groups of nuclear envelope antigens have been identified using monoclonal antibodies. On immunoblots the antigens correspond to distinct sets of polypeptides in the 175 X 10(3) molecular weight range. The antigens are enriched in a nuclear matrix-pore complex-lamina fraction of Drosophila tissue culture cells. We have studied the cellular distribution of these antigens throughout ooge...

fifty-seven patients suffering from systemic lupus erythematosus with different clinical features were studied from various aspects of immunological abnormalities, including anti-nuclear antibodies; anti-dna; anti-sm (smith's antigen); and, other extractable nuclear antigens. the tests employed were microhemagglutination, counter immunoelecterphoresis, and radio immunoassay (ria). the compariso...

We obtained a monoclonal antibody (RL13) that identifies three integral membrane proteins specific to the nuclear envelope of rat liver, a major 75-kD polypeptide and two more minor components of 68 and 55 kD. Immunogold labeling of isolated nuclear envelopes demonstrates that these antigens are localized specifically to the inner nuclear membrane, and that the RL13 epitope occurs on the inner ...

Inactive nuclei of chick erythrocyte resume RNA synthesis and increase in volume and dry mass in heterokaryons made by virus-induced fusion of human tumor cells (HeLa) with chick erythrocytes. Nuclear growth is due primarily to migration of human macromolecules into the chick nucleus. Human nucleoplasmic antigens were detected in the nucleoplasm and human nucleolar antigens were detected in the...

Precipitating antigens from Theileria parva have been partially purified. Two antigens from each of the schizont and piroplasm stages of the parasite were identified; the major antigens from the two stages shared the same specificity. The antigens showed considerable molecular heterogeneity, almost certainly a result of the preparative method, and they always contained large amounts of DNA. The...

mAbs raised against the human nuclear matrix (anti-NM)1 mAbs have been used to investigate the role of nuclear matrix antigens in pre-mRNA processing. The three anti-NM mAbs used in this study recognize antigens that are highly localized to nuclear matrix speckles. Surprisingly, all three of these mAbs preferentially immunoprecipitate splicing complexes containing exon sequences. The anti-NM mA...

Introduction Juvenile-onset Systemic Lupus Erythematosus (JSLE) is a multisystem autoimmune disorder characterized by the overproduction of autoantibodies against nuclear selfantigens. Activated neutrophils may undergo cell death by NETosis, forming mesh-like structures termed Neutrophil extracellular traps (NETs). Comprising of DNA, histones and antimicrobial proteins, increasing evidence impl...

An enzyme linked immunosorbent assay (ELISA) for detecting antibodies against cytomegalovirus induced immediate early antigens and early antigens was developed using purified nuclear antigens and was compared with the indirect immunofluorescence test. The tests were comparable in their ability to detect positive and negative sera, and antibody titres determined by both assays were similar. The ...