OBJECTIVE To determine the characteristics of oligoclonal bands that are frequently detected by serum protein electrophoresis (SPEP) and immunofixation electrophoresis (IFE) after stem cell transplantation. METHODS We retrospectively analyzed 56 patients with multiple myeloma (MM) undergoing transplantation, and standard immunofixation electrophoresis was used to identify and quantify parapro...

The clinical diagnosis of definite multiple sclerosis is supported by abnormalities in the cerebrospinal fluid: variable mild pleocytosis and elevation of total protein, moderately elevated total IgG in most patients, and the almost invariable presence of discrete immunoglobulins after electrophoresis, the oligoclonal bands. The oligoclonal bands are non-specific, and are seen in most diseases ...

Oligoclonal bands in cerebrospinal fluid reflect local B-cell responses associated with various neuroinflammatory disorders. In opsoclonus-myoclonus syndrome, cerebrospinal fluid B-cell expansion was demonstrated, but no studies of oligoclonal bands are available. In a prospective case-control study of 132 children (103 with opsoclonus-myoclonus, 29 neurologic control subjects), cerebrospinal f...

Oligoclonal IgM bands restricted to cerebrospinal fluid are an unfavorable prognostic marker in MS, the most common demyelinating disease of the CNS. We have attempted to identify the B cell subpopulation responsible for oligoclonal IgM secretion and the specificity of these bands. In addition, we explored the relationship between specificity and disease evolution. Intrathecal B cell subpopulat...

1 Hospital Universitari i Politècnic La Fe, València, Spain 2 Department of Functional Biology, University of Valencia, Valencia, Spain 3 Department of Biochemistry and Molecular Biology, University of Valencia, and INCLIVA Biomedical Research Institute, Valencia, Spain 4 Multiple Sclerosis Laboratory, Department of Biomedicine, Prince Felipe Research Center, Valencia, Spain Figure 1: Immunodet...

Controversy exists regarding the definition of oligoclonal bands in cerebrospinal fluid (CSF) and whether CSF bands with corresponding bands in the serum should be disregarded in the interpretation. Much of this controversy results from not distinguishing between the sensitivities of different techniques used for these studies. Because the combination isoelectric focusing/silver staining is mor...

Larrea et al. recently reported that patients with multiple myeloma (MM) who achieve complete remission (CR) after induction chemotherapy with novel agents (glucocorticoids with bortezomib, lenalidomide, thalidomide, or bortezomib with thalidomide) are more likely to have the emergence of oligoclonal bands in peripheral blood. While only 11% of patients who received conventional chemotherapy de...

A new modification of polyacrylamide gel electrophoresis (PAGE) was applied to cerebrospinal fluid proteins from patients with multiple sclerosis (MS). The same spinal fluids were also examined by a cytological technique. Over 90% of patients with clinically definite or early probable or latent MS showed abnormal PAGE patterns in the form of oligoclonal gammaglobulin bands. Reactive (atypical, ...

We developed a sensitive peroxidase staining procedure to identify oligoclonal band specificity in subacute sclerosing panencephalitis by subjecting serum and cerebrospinal fluid to isoelectric focusing and immunofixation with measles virus. The gel was washed and stained with horseradish peroxidase-conjugated goat antihuman immunoglobulin G. The samples showed dark brown measles-specific oligo...

The emergence of oligoclonal bands is associated with a favorable outcome after autologous stem cell transplantation in multiple myeloma. The aim of this study was to determine the prevalence of immunoglobulin oligoclonality in 33 patients with multiple myeloma in complete remission achieved with primary therapy with either cytotoxic agents (n = 18, 54.5%) or new induction regimens incorporatin...