A modular approach has been developed for enzymatic ligation of peptides/proteins with oligonucleotides through the design tag phosphoramidites as adaptors, paving way towards streamlined production peptide/protein-oligonucleotide conjugates.

Abstract We demonstrate the fabrication of a new DNA sensor that is based on optical interactions occurring between oligonucleotide-coated NaYF 4 :Yb 3+ ;Er upconversion nanoparticles and two-dimensional dichalcogenide materials, MoS 2 WS . Monodisperse were functionalized with single-stranded endowing ability to interact surface materials via van der Waals leading subsequent quenching fluoresc...

Oligonucleotide phosphorothioates (OPS) are DNA or RNA mimics where one phosphate oxygen is replaced by a sulfur atom. They have been shown to enter mammalian cells much more efficiently than non-modified DNA. Thus, solving of the key challenges with oligonucleotide technology, OPS became very useful in practice, several FDA-approved drugs on market late clinical trials. However, mechanism acco...

The interstrand photocrosslinking of a 5-chloro-2′-deoxy-4-thiouridine modified DNA oligonucleotide proceeds via two-step photocascade involving the formation thermally reversible crosslink and its conversion to stable fluorescent crosslink.

We identify protonation sites and study the competition of statistical fragmentation with localized bond cleavage after site-selective nitrogen 1s core-excitation by mass spectrometric detection gas-phase oligonucleotide cations.

A systematic study of the interactions between nucleases and oligonucleotide-coated gold nanoparticles (spherical nucleic acids, SNAs) demonstrates that modular units SNAs can be leveraged to either accelerate or suppress nuclease kinetics.

Gene therapy is in its development stage as a novel method for cancer treatment. Liposomes look promising as gene delivery vectors; however, investigations have shown that these vesicles are not doing well in some cases. It was decided here to investigate the possibility of augmentation of liposomal gene delivery by chemical penetration enhancers.Cationic liposome containing antisense oligonucl...