نتایج جستجو برای: one stepone tube pcr
تعداد نتایج: 2220314 فیلتر نتایج به سال:
The aim of the study was to present the usefulness of one tube reverse transcriptase polymerase chain reaction assay (RT-PCR) in comparison to virus isolation test (VI) in the detection of equine arteritis virus. The group of 82 semen samples taken from different stallions was examined. Equine arteritis virus was isolated in RK-13 cell monolayers from the seminal plasma of 11 animals. The same ...
tomato is an important vegetable crop in iran. recently symptoms associated with phytoplasma disease were observed in tomato cultivars in karaj vicinity. the phytoplasmas were detected and characterized using single-tube nested pcr and rflp. the universal primer pairs amplified the target dna with expected sizes from symptomatic samples in direct pcr and single-tube nested pcr. the patterns of ...
Traditional real-time polymerase chain reaction (PCR) requires a purified DNA sample for PCR amplification and detection. This requires PCR tests be conducted in clean laboratories, and limits its applications for field tests. This work developed a method that can carry out DNA purification, amplification and detection in a single PCR tube. The polypropylene PCR tube was first treated with chro...
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