Gram-negative bacteria produce outer membrane vesicles (OMVs) that package and deliver proteins, small molecules, and DNA to prokaryotic and eukaryotic cells. The molecular details of OMV biogenesis have not been fully elucidated, but peptidoglycan-associated outer membrane proteins that tether the outer membrane to the underlying peptidoglycan have been shown to be critical for OMV formation i...

BACKGROUND Pseudomonas aeruginosa is an important lifethreatening nosocomial pathogen which plays a prominent role in wound infections in burns patients. We designed this study to identify the isolates of P. aeruginosa recovered from burns patients at the genus and species levels by means of primers targeting oprI and oprL genes. METHODS During a 5-month period, wound samples were taken from ...

Pseudomonas aeruginosa is an important life-threatening nosocomial pathogen that plays a prominent role in wound infections of burned patients. We designed this study to identify the isolates of P. aeruginosa recovered from burned patients at the genus and species level through primers targeting oprI and oprL genes, and analyzed their antimicrobial resistance pattern. Over a 2-month period, wou...

The conjugation of antigens with ligands of pattern recognition receptors (PRR) is emerging as a promising strategy for the modulation of specific immunity. Here, we describe a new Escherichia coli system for the cloning and expression of heterologous antigens in fusion with the OprI lipoprotein, a TLR ligand from the Pseudomonas aeruginosa outer membrane (OM). Analysis of the OprI expressed by...

Vaccination management in children and adults with mastocytosis G. Zanoni , R. Zanotti, D. Schena, C. Sabbadini, R. Opri and P. Bonadonna Immunology Unit, Azienda Ospedaliera Universitaria Integrata, Multidisciplinary Outpatient Clinics for Mastocytosis, Azienda Ospedaliera Universitaria Integrata, Haematology Unit, Azienda Ospedaliera Universitaria Integrata, Dermatology Unit, Azienda Ospedali...

background and objectives: pseudomonas aeruginosa possesses a variety of virulence factors that may contribute to its pathogenicity. the aim of this study was to evaluate opri , oprl and toxa genes for pcr identification of clinical p. aeruginosa . in order to find out any relation between special virulence factors and special manifestation of p. aeruginosa infections, we detected virulence fac...