Burkholderia species are widely distributed in the environment. A Burkholderia zhejiangensis strain was isolated from pesticide-contaminated soil from an agricultural field in Mexico and identified as an organophosphorus compound-degrading bacterium. In this study, we report the draft genome sequence of Burkholderia zhejiangensis strain CEIB S4-3.

A versatile gene-fusion technique for immobilizing and visualizing biologically active enzymes which includes from the N to C-termini, an affinity histidine tag, the green fluorescent protein (GFP), a proteolytic enzyme (enterokinase, EK) cleavage site and the enzyme of interest, were developed. Specifically, the organophosphorus hydrolase was bound to the affinity (His(6))-reporter(GFP)-EK fus...

The objective of selecting strains Bacillus sp. has high activity in carbon metabolism for peat treatment, firstly focusing on the ability to degrade lignin, cellulose, phosphorus and protein. From gene pool, by surveying microbial capable degrading carbon-rich substrates including insoluble phosphate, proteolytic, we have identified two contain polyfermenticus subtilis strong metabolize decomp...

This paper reports a first microbial biosensor for rapid and cost-effective determination of organophosphorus pesticides fenitrothion and EPN. The biosensor consisted of recombinant PNP-degrading/oxidizing bacteria Pseudomonas putida JS444 anchoring and displaying organophosphorus hydrolase (OPH) on its cell surface as biological sensing element and a dissolved oxygen electrode as the transduce...

In the present work, six bacterial isolates capable of degrading chlorpyrifos (an organophosphorus pesticide) were obtained by enrichment from soil samples collected from the cultivated fields of Rajkot district of Gujarat State. The isolates were capable of growing in the presence of chlorpyrifos, in the concentration range of 60-100 mg/L. The highest value of MIC (100 mg/L) was reported for b...

Although microbial communities have varying degrees of exposure to environmental stresses such as chemical pollution, little is known on how these communities respond to environmental disturbances and how past disturbance history affects these community-level responses. To comprehensively understand the effect of organophosphorus insecticide application on microbiota in soils with or without in...