نتایج جستجو برای: oskm
تعداد نتایج: 67 فیلتر نتایج به سال:
BACKGROUND Somatic cells could be reprogrammed to induced pluripotent stem cells (iPS) by ectopic expression of OCT4, SOX2, KLF4 and MYC (OSKM). We aimed to gain insights into the early mechanisms underlying the induction of pluripotency. METHODS GSE28688 containing 14 gene expression profiles were downloaded from GEO, including untreated human neonatal foreskin fibroblasts (HFF1) as control,...
Expression of the four transcription factors Oct4, Sox2, Klf4, and c-Myc (OSKM) is sufficient to reprogram somatic cells into induced pluripotent stem (iPSCs). However, this process is slow and inefficient compared with the fusion of somatic cells with embryonic stem cells (ESCs), indicating that ESCs express additional factors that can enhance the efficiency of reprogramming. We had previously...
The male germinal lineage, which is defined as unipotent, produces sperm through spermatogenesis. However, embryonic primordial germ cells and postnatal spermatogonial stem cells (SSCs) can change their fate and convert to pluripotency in culture when they are not controlled by the testicular microenvironment. The mechanisms underlying these reprogramming processes are poorly understood. Testic...
By forcing the expression of certain genes, researchers can prompt non-reproductive cells to transform into early embryonic cells capable of giving rise to various cell types, a phenomenon known as induced pluripotency. Koji Tanabe et al. (pp. 12172–12179) circumvented the inefficiency of this process by revealing how the forced expression of four genes—OCT3/4, SOX2, KLF4, and c-MYC (OSKM)—can ...
Studies of human germ cell development are limited in large part by inaccessibility of germ cells during development. Moreover, although several studies have reported differentiation of mouse and human germ cells from pluripotent stem cells (PSCs) in vitro, differentiation of human germ cells from PSCs in vivo has not been reported. Here, we tested whether mRNA reprogramming in combination with...
Induced pluripotent stem (iPS) cells were first generated by forced expression of transcription factors (TFs) in fibroblasts. Recently, iPS cells have been generated more rapidly and efficiently using miRNAs with or without other transcription factors. However, the specific and collaborative roles of miRNAs and transcription factors in pluripotency acquisition and maintenance remain to be furth...
چکیده: زمینه و هدف: سلول های بنیادی پرتوان القایی induced pluripotent stem cells= ipsc))، سلول های اولیه و تمایز نیافته ای هستند که قادر به ایجاد تقریباً هر نوع سلولی در بدن می باشند. هدف از پژوهش حاضر تولید و استفاده مؤثر از وکتور لنتی ویروسی teto-fuw-oskm جهت انتقال ژن ها به سلول های فیبروبلاست انسانی ((hdfs= human fibroblast cells و در نهایت ارزیابی عملکرد این وکتور بود. روش بررسی: در این مطا...
Induced pluripotent stem cells (iPSCs) are commonly generated by transduction of Oct4, Sox2, Klf4, and Myc (OSKM) into cells. Although iPSCs are pluripotent, they frequently exhibit high variation in terms of quality, as measured in mice by chimera contribution and tetraploid complementation. Reliably high-quality iPSCs will be needed for future therapeutic applications. Here, we show that one ...
Fibroblasts can be reprogrammed to induced pluripotent stem cells (iPSCs) by application of transcription factors octamer-binding protein 4 (Oct4), SRY-box containing gene 2 (Sox2), Kruppel-like factor 4 (Klf4), and c-Myelocytomatosis oncogene (c-Myc) (OSKM), but the underlying mechanisms remain unclear. Here, we report that exogenous Oct4 and Sox2 can bind at the promoter regions of mir-141/20...
S omatic cells derived from differentiated tissues can be reprogrammed back to a pluripotent state. However, multiple barriers exist to prevent such extreme dedifferentiation from occurring in vivo, which explains the inefficient induction of pluripotency by ectopic coexpression of Oct4, Sox2, Klf4, and c-Myc (OSKM) in somatic cells. In PNAS, Ganier et al. (1) report a method that uses frog egg...
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