Objective(s): The prognosis of osteoporosis is very poor, and it is very important to identify a biomarker for prevention of osteoporosis. In this study, we aimed to identify candidate markers in osteoporosis and to investigate the role of candidate markers in osteogenic differentiation. Materials and Methods: Using Weighted Gene Co-Expression Network analysis, we identified three hub genes mig...

background: the mechanism of cadmium induced osteoporosis is not well understood, so in this study, we examined the toxicity of bone marrow mesenchymal stem cell (mscs) following treatment of rats with cdcl2 in drinking water, to revile the effect of this chemical on differentiation potential of mscs. methods: at the end of third passage, mscs were grown in the osteogenic medium for 21 days. to...

background periodontium may be able to respond to injuries by regeneration via the function of stem cells. objectives this study sought to assess the differentiation of human periodontal ligament stem cells (pdlscs) into osteoblasts in standard osteogenic medium and in a medium supplemented with 1,25-dihydroxyvitamin d3 (calcitriol). materials and methods in this experimental study, pdlscs were...

Staphylococcus aureus is the bacterial pathogen which is responsible for approximately 80% of all cases of human osteomyelitis. It can invade and remain within osteoblasts. The fate of intracellular Staph. aureus after the death of the osteoblast has not been documented. We exposed human osteoblasts to Staph. aureus. After infection, the osteoblasts were either lysed with Triton X-100 or trypsi...

Limited information is available on the role of MAPK phosphatase 1 (MKP1) signaling in osteoblasts. We have recently reported distinct roles for MKP1 during osteoblast proliferation, differentiation, and skeletal responsiveness to parathyroid hormone (PTH). As MKP1 regulates the phosphorylation status of MAPKs, we investigated the involvement of P-ERK and P-p38 MAPKs in MKP1 knockout (KO) early...

objectives: periodontal tissue regeneration for treatment of periodontal disease has not yet been mastered in tissue engineering. stem cells, scaffold, and growth factors are the three main basic components of tissue engineering. periodontal ligament (pdl) contains stem cells; however, the number, potency and features of these cells have not yet been understood. this study aimed to isolate and ...

objective: osteoblasts arise from multipotent mesenchymal stem cells (mscs) present in the bone marrow stroma and undergo further differentiation to osteocytes or bone cells. many factors such as proteins present in the wnt signaling pathway affect osteoblast differentiation. ror2 is an orphan tyrosine kinase receptor that acts as a co-receptor in the non-canonical wnt signaling pathway. howeve...

Osteoporosis is a common disease causing fracture in older populations. Abnormal apoptosis of osteoblasts contributes to the genesis of osteoporosis. Inhibiting apoptosis of osteoblasts provides a promising strategy to prevent osteoporosis. The proliferation of osteoblasts isolated from osteoporotic patients or healthy subjects was determined by MTT assay. Apoptosis was determined by Annexin V/...

Osteoarthritis (OA) is characterized by articular cartilage degradation and hypertrophic bone changes with osteophyte formation and abnormal bone remodeling. Two groups of OA patients were identified via the production of variable and opposite levels of prostaglandin E2 (PGE2) or leukotriene B4 (LTB4) by subchondral osteoblasts, PGE2 levels discriminating between low and high subgroups. We stud...

There is an increasing body of indirect evidence that suggests that the localization and activity of osteoclasts, the major agents of bone resorption, are controlled by osteoblasts. In this paper I provide direct evidence that osteoblasts are indeed able to alter the behaviour of osteoclasts. I used calcitonin (CT) to suppress the cytoplasmic activity of osteoclasts isolated on glass or plastic...