Allergic airway inflammation is attenuated by oral tolerization (oral exposure to allergen, followed by conventional sensitization and challenge with homologous antigen), which decreases airway allergen challenge-induced eosinophilic infiltration of the lungs and bone marrow eosinophilia. We examined its effects on bone marrow eosinophil and neutrophil production. Mice of wild type (BP-2, BALB/...

Background: The events that trigger type 2 immune responses are not well characterized. Given the central role of sweat in maintaining skin hydration, sweating disturbance at points allergen entry would predispose to food sensitization. A previous study demonstrated epicutaneously applied hapten more readily penetrates stratum corneum with a lower water content than higher water. Thus, is likel...

BACKGROUND Modified vaccinia virus Ankara (MVA)-encoding antigens are considered as safe vaccine candidates for various infectious diseases in humans. Here, we investigated the immune-modulating properties of MVA-encoding ovalbumin (MVA-OVA) on the allergen-specific immune response. METHODS The immune-modulating properties of MVA-OVA were investigated using GM-CSF-differentiated BMDCs from C5...

Objective(s): Human and animal studies have shown a close relationship between obesity and asthma severity. Here, we examined the effects of diet-induced obesity (DIO) on the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA in male Wistar rats tracheal after sensitization with ovalbumin (OVA). Materials and Methods:Twenty male Wistar rats divided to four groups, included, control group with ...

ContextSaikosaponin D (SSD) is a commonly prescribed agent against inflammatory diseases in Asian countries. However, the anti-allergic effect of SSD allergic rhinitis (AR) model not well known.ObjectiveWe investigated and anti-inflammatory effects on ovalbumin (OVA)-induced AR model.Materials methodBALB/c mice were divided into control, OVA, OVA + SSD, dexamethasone (Dex) groups. was establish...

We tested the hypothesis that atopy and/or allergic lung inflammation enhances alpha1-adrenoceptor-mediated contractions of the bronchial artery. Bronchial arterial resistance vessels were isolated from rabbits that had undergone either systemic ovalbumin (OVA) sensitization followed by saline aerosol challenge (OVA/saline rabbits), or OVA sensitization followed by OVA aerosol challenge (OVA/OV...

Immunoglobulins (Ig), particularly IgE, are believed to be crucially involved in the pathogenesis of asthma and, equally, in allergic models of the disease. To validate this paradigm we examined homozygous mutant C57BL/6 mice, which are B cell deficient, lacking all Ig. Mice were immunized intraperitoneally with 10 micrograms ovalbumin (OVA) plus alum, followed by daily (day 14-20) 30 min expos...

The aim of the present study was to investigate whether glycated ovalbumin (OVA) showed novel activity at the lipid-water interface. Mannosylated OVA (Man-OVA) was prepared by modification of the carboxyl groups with p-aminophenyl α-dextro (d)-mannopyranoside. An increase in the number of modified carboxyl groups increased the membrane-damaging activity of Man-OVA on cell membrane-mimicking ves...

Oral administration of soluble antigen can induce peripheral tolerance to the antigen. This study was conducted to evaluate whether gamma-irradiated ovalbumin (OVA) can induce oral tolerance. To investigate this, we administrated intact or irradiated OVA to mice, induced allergic response using intact OVA and alum, then compared humoral and cellular immune responses. Mice treated with gammairra...

Mol. Nutr. Food Res. 2023, 67, 202200221 DOI: 10.1002/mnfr.202200221 Epidemiologic evidence suggests a link between food allergy and the intake of advanced glycation end-products (AGEs). This study investigated role allergen-specific non-specific AGEs in allergenicity ovalbumin (OVA) mouse model allergy. The OVA-AGE elicits stronger Th2-response than native OVA, comparison to severer disruption...