An amperometric microbial biosensor for highly specific, sensitive and rapid quantitative determination of p-nitrophenol was developed. The biosensor takes advantage of the ability of Moraxella sp. to specifically degrade p-nitrophenol to hydroquinone, a more electroactive compound than p-nitrophenol. The electrochemical oxidation current of hydroquinone formed in biodegradation of p-nitropheno...

1. Glucuronide formation of bilirubin and p-nitrophenol in vitro with excess of UDP-glucuronic acid by UDP-glucuronyltransferase from livers of young and adult rabbits was studied. 2. The development of UDP-glucuronyltransferase for the two substrates followed a markedly different pattern during maturation of young rabbits, p-nitrophenol-conjugation ability being much higher at birth than that ...

Cyclodextrin (CD) capped CdTe quantum dots (QDs) were prepared with uniform dimension (average diameter ∼5 nm) and high quantum yield (ca. 65%). By taking advantage of the inclusion complexation of CD, β-CD-CdTe QDs exhibited strong fluorescence quenching in a linear relationship with the concentration of o-, m- and p-nitrophenol in the range of 20-100 μM. The detection limit reached 0.05 μM fo...

The relationship between the intraluminal Ca2+ content of endoplasmic reticulum and the rate of the glucuronidation of p-nitrophenol was investigated in isolated rat hepatocytes. Different agents which decrease the Ca2+ level in the endoplasmic reticulum [calcium ionophores (A23187, ionomycin) or Ca(2+)-ATPase inhibitors(thapsigargin,2,5-di-(t-butyl)-1,4-benzohydroquinone+ ++)] inhibited the co...

The mammalian glucoside-conjugation pathway was studied by using p-nitrophenol as the model substrate and mouse liver microsomal preparations as the source of enzyme. The microsomal preparations supplemented with UDP-glucose glucosylated p-nitrophenol; p-nitrophenyl glucoside was identified by chromatography in six solvent systems. The unsolubilized glucosyltransferase of fresh microsomal prepa...

biodegradation kinetics of 4-nitrophenol (pnp) in aqueous solution by a gram negative soil bacterium, ralstoniaeutropha was firstly studied in a small scale batch reactor. the degradation of pnp was evaluated at initial pnp concentrations ranging from 3 mg/l to 14 mg/l. the rate of pnp consumption by the bacterium culture was modeled using monod and contois kinetics in batch condition. pnp degr...

4 Nitrophenol (NP) or para nitrophenol (PNP) is one of the emerging pollutants from pesticides industries and it a major discharge any phenol consuming industry also severs as pharmaceutical wastewater. Design development photocatalytic treatment p-nitrophenol particularly to suit recovery reuse catalyst will aid in its commercial scale development. P-nitrophenol degradation has been studied ce...