The combination of cytoplasmic male sterility (CMS) in one parent and a restorer gene (Rf) to restore fertility in another are indispensable for the development of hybrid varieties. To genotype rice lines at the restoring fertility (Rf) loci, 38 lines were crossed with a sterile tester (rfrf) line. Pollen fertility test was performed to identify sterile and fertile F1 hybrids. Seven lines were ...

An experiment was conducted at Bangladesh Rice Research Institute (BRRI), Gazipur during T.Aman season of 2007 with a view to evaluating seven maintainer and 30 restorer lines for yield and yield contributing characters for selecting promising maintainer and restorer lines for developing rice hybrids in our local environment. Significant variations were noticed among the parental lines for all ...

Cotton is the most important commercial crop of India and heterosis in cotton is well documented with 7 to 50 per cent heterosis in interspecific (G. hirsutum x G. barbadense) hybrids, 10 to 138 per cent in intra-hirsutum hybrids and upto 220 per cent in desi (G. herbaceum x G. arboreum) hybrids. Currently hybrid cotton, especially Bt cotton hybrids, occupies more than 80 per cent of cultivated...

The Polima system of cytoplasmic male sterility (Pol CMS) was widely used for hybrid seed production, but with high probability occurrence maternal false due to the fertility CMS line greatly affected by ambient temperature, therefore, detection hybrids is key purity identification. In present study, SSR markers were designed based on flanking sequences Pol restorer gene  Rfp , and applica...

Rice restorer lines play an important role in three-line hybrid rice production. Previous research based on molecular tagging has suggested that the restorer lines used widely today have narrow genetic backgrounds. However, patterns of genetic variation at a genome-wide scale in these restorer lines remain largely unknown. The present study performed re-sequencing and genome-wide variation anal...

Predicted climate changes, in particular, the increased dimension and frequency of heat waves, are expected to affect crop growth in the future seriously. Hybrid rice relies on seed production from male sterile and restorer lines. Experiments were conducted over two consecutive years to compare the high temperature tolerance of parents of different hybrid rice combinations, in terms of fertilit...

Restoration of pollen fertility to cytoplasmic male sterile (CMS) Phaseolus vulgaris by a nuclear restorer gene provides a system for studying nuclear-cytoplasmic interactions. Introduction of a nuclear restorer gene to this CMS line of P. vulgaris (CMS-Sprite) results in a mitochondrial genome rearrangement similar to that observed upon spontaneous cytoplasmic reversion to fertility. Three spo...

for different agronomic traits in sunflower (helianthus annuus l.) inbred lines were estimated using “line × tester” analysis method. six new restorer lines were crossed with four cms lines as tester in 2006 growing season. the 24 f1 single cross combinations were planted in complete randomized block design with four replications in khoy field research station in 2007 growing season. there were...

NADP-dependent isocitrate dehydrogenase (NADP-ICDH) is an important enzyme involved in energy metabolism. The complete coding sequence of the pepper (Capsicum annuum) NADP-ICDH gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. Nucleotide sequence analysis reveal...

the present study was carried out with the objective of validating linked ssr markers to rf genes and adopting marker assisted selection (mas) for restorer/non-restorer line detection in wild abortive (wa) type of cytoplasmic male sterility (cms). twelve ssr markers reported to be linked to rf genes were analyzed in the mapping population of nedaa/pajouhesh. among these, three markers, namely, ...