background: toxoplasmosis is a zoonotic disease with high seroprevalence worldwide. several immunological methods have been described for diagnosis of toxoplasmosis. objective: to determine the parasitemia period in patients infected with toxoplasma using pcr and comparing serological data with molecular results. methods: 154 serum samples from patients with toxoplasmosis were examined. presenc...

Background: Toxoplasmosis is a zoonotic disease with high seroprevalence worldwide. Several immunological methods have been described for diagnosis of toxoplasmosis. Objective: To determine the parasitemia period in patients infected with toxoplasma using PCR and comparing serological data with molecular results. Methods: 154 serum samples from patients with toxoplasmosis were examined. Presenc...

The principal objective of this study was to investigate the prevalence of toxoplasmosis in household and stray cats in Seoul, Republic of Korea. We collected blood samples from 72 stray and 80 household cats, and all samples were examined by ELISA and nested PCR. The overall positive rates of Toxoplasma gondii in stray cats were 38.9% (28/72), with 15.3% (11/72) in ELISA and 30.6% (22/72) in P...

AIMS To compare the culture and PCR methods for detection of Brucella melitensis in blood and lymphoid tissue samples obtained from slaughtered sheep (n = 162) testing positive/negative in serological tests (Rose Bengal test and serum agglutination test). METHODS AND RESULTS Of 162 sheep examined, 45 were positive and 117 negative in serological tests. A PCR assay based on a pair of Br. melit...

Few studies have examined the molecular epidemiology of cryptosporidiosis in developing countries. In this study, DNA of 69 microscopy-positive human fecal samples collected from Malawi were examined by multilocus genetic analyses. From 43, 27 and 28 of the samples, the SSU rRNA, 70 kDa heat shock protein (HSP70) and 60 kDa glycoprotein (GP60) genes, respectively, were successfully PCR-amplifie...

INTRODUCTION Bacteria and/or their antigens have been implicated in the pathogenesis of reactive arthritis (ReA). Several studies have reported the presence of bacterial antigens and nucleic acids of bacteria other than those specified by diagnostic criteria for ReA in joint specimens from patients with ReA and various arthritides. The present study was conducted to detect any bacterial DNA and...

BACKGROUND Giardia lamblia is one of the most prevalent intestinal flagellate protozoa that infects a wide range of vertebrate hosts causing severe intestinal disorder in children.This study was performed to determine subspecies of G.lamblia by the PCR-RFLP method, targeting the glutamate dehydrogenase(gdh)locus, in hospitalized children at Urmia Mutahhari Hospital, West Azerbaijan Province,Ira...

Background and Aims: The aim of present study was to investigate the molecular characteristic of Staphylococcus aureus to detect mec A gene and to type SCCmec in strain isolated from healthy worker at Larestan Hospital. Material and Methods: This study was carried out from the 250 nasal swab healthy worker  at Larestan hospital. Multiplex polymerase chain reaction for mecA gene was perf...