In article number 2100430 by Sung Gap Im, Kyoung G. Lee, and co-workers, an “all-in-one tube” is devised coating poly-(2-dimethylaminomethyl styrene) (pDMAMS) on a polymerase chain reaction (PCR) tube via initiated chemical vapor deposition (iCVD) to extract, amplify, detect pathogens within single tube. The all-in-one can capture the DNA of charge interaction directly perform real-time PCR, re...

Screening Feline Blood Donors for Bartonella henselae Infection: Comparison between Indirect Immunofluorescent Antibody Test (IFAT) and Polymerase Chain Reaction (PCR) Results Eva Spada1, Daniela Proverbio1, Roberta Perego1, Ilaria Canzi1, Luciana Baggiani1, Claudia De Maria2, Francesca Marino2, Santo Caracappa2 1Department of Veterinary Medicine, Veterinary Transfusion Unit (REV), University o...

The polymerase chain reaction (PCR) is a powerful and widely used technique that has greatly advanced our ability to analyze genes. Genomic deoxyribonucleic acid (DNA) present in cells contains many thousands of genes. This makes it difficult to isolate and analyze any individual gene. PCR allows specific DNA sequences, usually corresponding to genes or parts of genes, to be copied from genomic...

1. Introduction The polymerase chain reaction (PCR) is a powerful method for fast in vitro enzymatic amplifications of specific DNA sequences. PCR amplifications can be grouped into three different categories: standard PCR, long PCR, and multiplex PCR. Standard PCR involves amplification of a single DNA sequence that is less than 5 kb in length and is useful for a variety of applications, such ...

Polysaccharides, secondary metabolites and poly-phenolics are known to co-isolate with nucleic acids from plant tissues resulting in inhibition of molecular manipulations. RNA isolated from the polyphenolic-rich resurrection plant, Myrothamnus flabellifolius, was demonstrated to inhibit a standard polymerase chain reaction used as an assay despite the inclusion of the polyphenolic-binding compo...

UNLABELLED This study observed the quantity and functions of wound neu- trophils after topical insulin treatment and investigated the effect of insulin on the wound inflammatory response, as well as the mechanism of insulin-induced wound healing. METHODS Full-thickness excisional wounds were made on the dorsal symmetrical site of C57BL/6J mice. The wounds were treated with either 0.03U insuli...

conclusions l. innocua is not a pathogen, but the presence of the bacterium could be an indicator of probable contamination with l. monocytogenes. moreover, there is a potential risk to public health from the consumption of raw or undercooked burgers, which may increase the possibility of the acquisition of resistance to antibiotics. objectives the aim of this study was to evaluate the occurren...