Viral diseases are the most serious problem in shrimp culture as they result in severe production and economic losses. Monodon baculovirus (MBV) is a member of nucleopolyhedrosis virus (NPV) group that infect penaeid shrimps. Polymerase chain reaction (PCR) assays have been reported for specific and accurate detection of MBV. As variations in the strains of MBV would greatly affect the detectio...

Four species of penaeid prawn cultured in Australia (Penaeus monodon, Penaeus esculentus, Marsupenaeus japonicus and Fenneropenaeus merguiensis) were injected with a virulent preparation of gill-associated virus (GAV). P. monodon (average weight = 8.9, 13.9 and 19.2 g), P. esculentus (average weight = 19.5 g), F. merguiensis (average weight = 10.5 g), and small (average weight = 5.8 g) M. japon...

Seafood constitutes an important food component and a cheap source of protein in developing countries. Shrimps are prone to bacterial contamination in their environment and have been frequently implicated in Vibrio parahaemolyticus infection. Of the 121 Penaeus monodon collected from coastal beaches in Douala, 79(65.3%) were contaminated with Vibrio spp. Presumed Vibrio sp. were identified by s...

A study was undertaken to determine the most effective element among the commonly used antimicrobial agents, especially in the body muscle of black tiger shrimp (Penaeus monodon) and giant freshwater prawn (Macrobrachium rosenbergii) on the basis of different chemical interventions. Using 10 ppm calcium hypochlorite, the microbial load has been reduced more significantly (p>0.001) than trisodiu...

We describe a duplex real-time PCR assay using TaqMan probes for the simultaneous detection of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV). Both MBV and HPV are shrimp enteric viruses that infect intestinal and hepatopancreatic epithelial cells. Both viruses can cause significant mortalities and depressed growth in infected larval, postlarval, and early juvenile stages of sh...

Yellow head virus infects cultured shrimps and causes severe mortality resulting in a great economic loss. Haemolymph injection of dsRNA(pro) corresponding to the protease motif of YHV genome resulted in a complete inhibition of YHV replication. The effect of dsRNA lasted for at least 5 days. Injecting sequence-unrelated dsRNA(gfp) or dsRNA(TSV-pol) also resulted in an inhibition of YHV replica...

Translationally controlled tumour proteins (TCTP) cDNA were cloned from the haemolymph of Scylla paramamosain by reverse transcription PCR using primers derived from Penaeus monodon. Nucleotide sequence analysis revealed 507 bp open reading frame (ORF) encoding 168 amino acid residues with a predicted molecular mass and pI of approximately 19.2 kDa and 4.5, respectively. Interestingly, mud crab...

Prevalence status of white spot disease (WSD), caused by white spot syndrome virus (WSSV) and Monodon baculovirus (MBV), was studied in different prawn farms in North and South-24 paragana districts of West Bengal, specifically in the areas previously affected by WSD. In total 283 samples of giant tiger prawn Penaeus monodon, Indian white prawn P. indicus and giant freshwater prawn Macrobrachiu...

Bacteria phenotypically resembling members of the phylogenetically distinct planctomycete group of the domain Bacteria were isolated from postlarvae of the giant tiger prawn, Penaeus monodon. A selective medium designed in the light of planctomycete antibiotic resistance characteristics was used for this isolation. Planctomycetes were isolated from both healthy and monodon baculovirus-infected ...